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Literature summary for 1.10.3.4 extracted from

  • Jia, H.; Gao, Z.; Ma, Y.; Zhong, C.; Wang, C.; Zhou, H.; Wei, P.
    Preparation and characterization of a highly stable phenoxazinone synthase nanogel (2016), Chem. Cent. J., 10, 34 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.4

Inhibitors

Inhibitors Comment Organism Structure
DMSO the native enzyme shows less than 5% residual activity in the presence of DMSO Streptomyces antibioticus
ethanol the native enzyme shows less than 5% residual activity in the presence of ethanol Streptomyces antibioticus
ethyl acetate the native enzyme shows less than 5% residual activity in the presence of ethyl acetate Streptomyces antibioticus
methanol the native enzyme shows less than 5% residual activity in the presence of methanol Streptomyces antibioticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 2-Aminophenol enzyme on nanogel, at pH 4.5 and 25°C Streptomyces antibioticus
0.077
-
 2-Aminophenol free enzyme, at pH 4.5 and 25°C Streptomyces antibioticus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme is a multi-copper oxidase Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminophenol + O2 Streptomyces antibioticus
-
 2-aminophenoxazin-3-one + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces antibioticus Q53692
-
-

Storage Stability

Storage Stability Organism
4°C, enzyme on nanogel, 5 weeks, no loss of activity Streptomyces antibioticus
4°C, lyophilized enzyme, 5 weeks, 65% loss of activity Streptomyces antibioticus
4°C, native enzyme at pH 4.5, 5 weeks, 98% loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces antibioticus ?
-
 ?
2-aminophenol + O2
-
 Streptomyces antibioticus 2-aminophenoxazin-3-one + H2O
-
 ?

Synonyms

Synonyms Comment Organism
phenoxazinone synthase
-
 Streptomyces antibioticus
PHS
-
 Streptomyces antibioticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 free enzyme Streptomyces antibioticus
50
-
 enzyme on nanogel Streptomyces antibioticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the native enzyme loses about 90% of its activity whereas the enzyme on nanogel loses about 50% of its activity after 2 h preincubation at 60°C Streptomyces antibioticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 enzyme on nanogel Streptomyces antibioticus
4.5
-
 free enzyme Streptomyces antibioticus