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Literature summary for 1.10.3.4 extracted from
- Phenoxazinone synthase: whats' in a name? (2009), Trends Biotechnol., 27, 248-258.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dimercaptopropanol | - |
Bauhinia monandra |  |
| 2-Aminophenol | inhibitory above 0.6 mM | Trametes coccinea | |
| 2-Nitrophenol | - |
Streptomyces griseus subsp. griseus | |
| 3-amino-4-hydroxybenzensulfonic acid | - |
Streptomyces griseus subsp. griseus | |
| 3-hydroxyanthranilic acid | - |
Bauhinia monandra | |
| 4-hydroxy-3-nitrobenzaldehyde | - |
Streptomyces griseus subsp. griseus | |
| 4-hydroxybenzaldehyde | - |
Streptomyces griseus subsp. griseus | |
| 8-hydroxyquinoline | - |
Streptomyces antibioticus | |
| Ag+ | - |
Bauhinia monandra | |
| alpha,alpha'-dipyridyl | - |
Streptomyces antibioticus | |
| aniline | - |
Streptomyces griseus subsp. griseus | |
| anthranilic acid | - |
Bauhinia monandra | |
| ascorbic acid | - |
Bauhinia monandra | |
| ascorbic acid | - |
Trametes coccinea | |
| Atebrin | - |
Bauhinia monandra | |
| Atebrin | - |
Tecoma stans | |
| azide | - |
Bauhinia monandra | |
| azide | - |
Streptomyces antibioticus | |
| Co2+ | - |
Tecoma stans | |
| Cu2+ | - |
Bauhinia monandra | |
| Cu2+ | - |
Streptomyces antibioticus | |
| CuSO4 | - |
Trametes coccinea | |
| cyanide | - |
Bauhinia monandra | |
| cyanide | - |
Streptomyces antibioticus | |
| cyanide | - |
Tecoma stans | |
| cysteine | - |
Bauhinia monandra | |
| cysteine | - |
Trametes coccinea | |
| EDTA | - |
Streptomyces antibioticus | |
| Fe2+ | - |
Bauhinia monandra | |
| Fe2+ | - |
Streptomyces antibioticus | |
| Fe3+ | - |
Tecoma stans | |
| FeSO4 | - |
Trametes coccinea | |
| glutathione | - |
Bauhinia monandra | |
| glutathione | - |
Trametes coccinea | |
| Hg2+ | - |
Bauhinia monandra | |
| Hg2+ | - |
Tecoma stans | |
| K+ | - |
Streptomyces antibioticus | |
| L-tyrosine | - |
Streptomyces griseus subsp. griseus | |
| Mg2+ | - |
Tecoma stans | |
| N-ethylmaleimide | - |
Bauhinia monandra | |
| N-ethylmaleimide | - |
Trametes coccinea | |
| Na3AsO3 | - |
Trametes coccinea | |
| NaBH4 | - |
Trametes coccinea | |
| NH4+ | - |
Streptomyces antibioticus | |
| p-chloromercuribenzoate | - |
Trametes coccinea | |
| p-hydroxymercuribenzoate | - |
Bauhinia monandra | |
| phenol | - |
Streptomyces griseus subsp. griseus | |
| Sodium diethyldithiocarbamate | - |
Streptomyces antibioticus | |
| Zn2+ | - |
Streptomyces antibioticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | GriF is a type 3 copper oxidase and contains two copper atoms | Streptomyces griseus subsp. griseus | |
| Cu2+ | the enzyme is a blue multicopper oxidase | Streptomyces antibioticus | |
| Mn2+ | required | Trametes coccinea | |
| Mn2+ | required | Tecoma stans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 2-aminophenol + 2 O2 | Streptomyces antibioticus | - |
isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | Trametes coccinea | - |
isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | Tecoma stans | - |
isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | Bauhinia monandra | - |
isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | Streptomyces griseus subsp. griseus | - |
isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2-amino-4-methylphenol + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| 3,4-dihydroxybenzaldehyde + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| 3-amino-4-hydroxybenzaldehyde + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| 3-hydroxyanthranilic acid + O2 | Streptomyces antibioticus | - |
2-amino-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid + H2O | - |
? | |
| 3-hydroxyanthranilic acid + O2 | Streptomyces griseus subsp. griseus | - |
2-amino-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid + H2O | - |
? | |
| 3-hydroxykynurenine + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| 4-methyl-3-hydroanthranilic acid methyl ester + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| 4-methyl-3-hydroxyanthranilic acid + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| catechol + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| catechol + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| ferrocyanide + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| L-DOPA + O2 | Streptomyces antibioticus | - |
? | - |
? | |
| L-DOPA + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| protocatechuic acid + O2 | Streptomyces griseus subsp. griseus | - |
? | - |
? | |
| thiophenol + O2 | Streptomyces antibioticus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bauhinia monandra | - |
- |
- |
| Streptomyces antibioticus | - |
- |
- |
| Streptomyces griseus subsp. griseus | - |
- |
- |
| Tecoma stans | - |
- |
- |
| Trametes coccinea | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 2-aminophenol + 2 O2 | - |
Streptomyces antibioticus | isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | - |
Trametes coccinea | isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | - |
Tecoma stans | isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | - |
Bauhinia monandra | isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2 2-aminophenol + 2 O2 | - |
Streptomyces griseus subsp. griseus | isophenoxazine + 2 H2O + H2O2 | - |
? | |
| 2-amino-4-methylphenol + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| 3,4-dihydroxybenzaldehyde + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| 3-amino-4-hydroxybenzaldehyde + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| 3-hydroxyanthranilic acid + O2 | - |
Streptomyces antibioticus | 2-amino-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid + H2O | - |
? | |
| 3-hydroxyanthranilic acid + O2 | - |
Streptomyces griseus subsp. griseus | 2-amino-3-oxo-3H-phenoxazine-1,9-dicarboxylic acid + H2O | - |
? | |
| 3-hydroxykynurenine + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| 4-methyl-3-hydroanthranilic acid methyl ester + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| 4-methyl-3-hydroxyanthranilic acid + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| catechol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| catechol + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| ferrocyanide + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| L-DOPA + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
| L-DOPA + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| protocatechuic acid + O2 | - |
Streptomyces griseus subsp. griseus | ? | - |
? | |
| thiophenol + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-aminophenol:oxygen oxidoreductase | - |
Streptomyces antibioticus |
| 2-aminophenol:oxygen oxidoreductase | - |
Trametes coccinea |
| 2-aminophenol:oxygen oxidoreductase | - |
Tecoma stans |
| 2-aminophenol:oxygen oxidoreductase | - |
Bauhinia monandra |
| 2-aminophenol:oxygen oxidoreductase | - |
Streptomyces griseus subsp. griseus |
| esophenoxazine synthase | - |
Streptomyces antibioticus |
| esophenoxazine synthase | - |
Trametes coccinea |
| esophenoxazine synthase | - |
Tecoma stans |
| esophenoxazine synthase | - |
Bauhinia monandra |
| esophenoxazine synthase | - |
Streptomyces griseus subsp. griseus |
| GriF | - |
Streptomyces griseus subsp. griseus |
| isophenoxazine synthase | - |
Streptomyces antibioticus |
| isophenoxazine synthase | - |
Trametes coccinea |
| isophenoxazine synthase | - |
Tecoma stans |
| isophenoxazine synthase | - |
Bauhinia monandra |
| isophenoxazine synthase | - |
Streptomyces griseus subsp. griseus |
| o-aminophenol oxidase | - |
Streptomyces antibioticus |
| o-aminophenol oxidase | - |
Trametes coccinea |
| o-aminophenol oxidase | - |
Tecoma stans |
| o-aminophenol oxidase | - |
Bauhinia monandra |
| o-aminophenol oxidase | - |
Streptomyces griseus subsp. griseus |
| o-aminophenol:O2 oxidoreductase | - |
Streptomyces antibioticus |
| o-aminophenol:O2 oxidoreductase | - |
Trametes coccinea |
| o-aminophenol:O2 oxidoreductase | - |
Tecoma stans |
| o-aminophenol:O2 oxidoreductase | - |
Bauhinia monandra |
| o-aminophenol:O2 oxidoreductase | - |
Streptomyces griseus subsp. griseus |
| oxidase, o-aminophenol | - |
Streptomyces antibioticus |
| oxidase, o-aminophenol | - |
Trametes coccinea |
| oxidase, o-aminophenol | - |
Tecoma stans |
| oxidase, o-aminophenol | - |
Bauhinia monandra |
| oxidase, o-aminophenol | - |
Streptomyces griseus subsp. griseus |
| phenoxazinone synthase | - |
Streptomyces antibioticus |
| phenoxazinone synthase | - |
Trametes coccinea |
| phenoxazinone synthase | - |
Tecoma stans |
| phenoxazinone synthase | - |
Bauhinia monandra |
| phenoxazinone synthase | - |
Streptomyces griseus subsp. griseus |
| PHS | - |
Streptomyces antibioticus |
| PHS | - |
Trametes coccinea |
| PHS | - |
Tecoma stans |
| PHS | - |
Bauhinia monandra |
| PHS | - |
Streptomyces griseus subsp. griseus |
| PhsA | - |
Streptomyces antibioticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bauhinia monandra |
| 42 | - |
- |
Streptomyces antibioticus |
| 45 | - |
- |
Tecoma stans |
| 55 | - |
- |
Streptomyces griseus subsp. griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.2 | - |
Streptomyces antibioticus |
| 6.2 | - |
- |
Tecoma stans |
| 6.2 | - |
- |
Bauhinia monandra |
| 8.5 | 10.5 | - |
Streptomyces griseus subsp. griseus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Tecoma stans | |
| additional information | no cofactor required | Bauhinia monandra | |
| riboflavin 5'-phosphate | - |
Trametes coccinea | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Streptomyces antibioticus | gene expression is subject to glucose repression | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | GriF plays a role in the biosynthesis of the antibiotic grixazone | Streptomyces griseus subsp. griseus |
| physiological function | PhsA plays a role in pigment production | Trametes coccinea |
| physiological function | PhsA plays a role in spore coat pigmentation and melanin production | Streptomyces antibioticus |
| physiological function | the enzyme plays a role in atechol production | Bauhinia monandra |
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