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Literature summary for 1.10.3.4 extracted from

  • Suzuki, H.; Furusho, Y.; Higashi, T.; Ohnishi, Y.; Horinouchi, S.
    A novel o-aminophenol oxidase responsible for formation of the phenoxazinone chromophore of grixazone (2006), J. Biol. Chem., 281, 824-833.
    View publication on PubMed
Search for more literature for 1.10.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)/pLysS cells Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
3-amino-4-hydroxybenzensulfonic acid
-
 Streptomyces griseus
4-hydroxy-3-nitrobenzaldehyde
-
 Streptomyces griseus
aniline
-
 Streptomyces griseus
L-tyrosine
-
 Streptomyces griseus
o-Nitrophenol
-
 Streptomyces griseus
p-Hydroxybenzaldehyde
-
 Streptomyces griseus
phenol
-
 Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 3,4-Dihydroxybenzaldehyde in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
0.58
-
 3-amino-4-hydroxybenzaldehyde in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
0.75
-
 2-amino-4-methylphenol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
3.5
-
 o-aminophenol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
5.5
-
 L-Dopa in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
19
-
 catechol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 Superdex 200 gel filtration Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Cu2+-charged HiTrap chelating HP column chromatography, HiTrap Q column chromatography and Resource PHE column chromatography Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000035
-
 crude cell extract, at 30°C Streptomyces griseus
0.066
-
 after 19000fold purification, at 30°C Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-methylphenol + O2
-
 Streptomyces griseus ?
-
 ?
2-amino-4-methylphenol + O2
-
 Streptomyces griseus IFO 13350 ?
-
 ?
3,4-dihydroxybenzaldehyde + O2
-
 Streptomyces griseus ?
-
 ?
3,4-dihydroxybenzaldehyde + O2
-
 Streptomyces griseus IFO 13350 ?
-
 ?
3-amino-4-hydroxybenzaldehyde + O2
-
 Streptomyces griseus ?
-
 ?
catechol + O2
-
 Streptomyces griseus ?
-
 ?
catechol + O2
-
 Streptomyces griseus IFO 13350 ?
-
 ?
L-DOPA + O2
-
 Streptomyces griseus ?
-
 ?
L-DOPA + O2
-
 Streptomyces griseus IFO 13350 ?
-
 ?
o-aminophenol + O2
-
 Streptomyces griseus 2-aminophenoxazin-3-one + H2O
-
 ?
protocatechuic acid + O2
-
 Streptomyces griseus ?
-
 ?
protocatechuic acid + O2
-
 Streptomyces griseus IFO 13350 ?
-
 ?

Synonyms

Synonyms Comment Organism
GriF
-
 Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 at pH 7.0 Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme remains active below 55°C at pH 7.0 for 20 min at various temperatures Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.066
-
 L-Dopa in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
0.8
-
 3,4-Dihydroxybenzaldehyde in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
12
-
 catechol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
14
-
 3-amino-4-hydroxybenzaldehyde in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
18
-
 2-amino-4-methylphenol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus
20
-
 o-aminophenol in 50 mM sodium phosphate, pH 7.0, 30°C Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10.5
-
 Streptomyces griseus

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Streptomyces griseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
 p-Hydroxybenzaldehyde
-
 Streptomyces griseus
3.2
-
 L-tyrosine
-
 Streptomyces griseus
3.9
-
 4-hydroxy-3-nitrobenzaldehyde
-
 Streptomyces griseus
6.1
-
 o-Nitrophenol
-
 Streptomyces griseus
14
-
 aniline
-
 Streptomyces griseus
21
-
 phenol
-
 Streptomyces griseus
31
-
 3-amino-4-hydroxybenzensulfonic acid
-
 Streptomyces griseus