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Literature summary for 1.10.3.4 extracted from
- Isophenoxazine synthase apoenzyme from Pygnoporus coccineus (1965), Biochim. Biophys. Acta, 96, 318-327.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apoenzyme | Trametes coccinea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | - |
Trametes coccinea |  |
| CuSO4 | - |
Trametes coccinea | |
| cysteine | - |
Trametes coccinea | |
| FeSO4 | - |
Trametes coccinea | |
| glutathione | - |
Trametes coccinea | |
| N-ethylmaleimide | - |
Trametes coccinea | |
| Na3AsO3 | - |
Trametes coccinea | |
| NaBH4 | - |
Trametes coccinea | |
| o-aminophenol | substrate inhibition above 0.6 mM | Trametes coccinea | |
| p-chloromercuribenzoate | - |
Trametes coccinea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.264 | - |
FMN | - |
Trametes coccinea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | holoenzyme reconstituted by addition of riboflavin and Mn2+ required for maximal activity, ineffective in absence of FMN | Trametes coccinea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes coccinea | - |
wood-rotting fungus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Trametes coccinea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Trametes coccinea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trametes coccinea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 2-aminophenol + 2 O2 | oxidation of o-aminophenol to o-quinoneimine and subsequent condensation of o-aminophenol to 2-amino-3H-isophenoxazin-3-one in absence of Mn2+ requires about 1 mol of riboflavin 5'-phosphate per mol of o-aminophenol oxidized, in presence of Mn2+ riboflavin 5'-phosphate acts as catalyst | Trametes coccinea | isophenoxazine + 2 H2O + H2O2 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | FAD no action as cofactor | Trametes coccinea | |
| riboflavin 5'-monophosphate | holoenzyme reconstituted by addition of riboflavin 5'-phosphate and Mn2+ | Trametes coccinea | |
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Release 2023.1 (January 2023)
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