Cloned (Comment) | Organism |
---|---|
expression of RNAi constructs in tomato plants using transfection of WVa106 tomato cotyledons via Agrobacterium strain GV3101, real-time quantitative PCR expression analysis | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of RNAi knockout plants lacking AAO activity, phenotype, overview. RNAi tomato lines have reduced ascorbate oxidase transcript and activity in leaves. Stomatal conductance is increased in transgenic ascorbate oxidase lines | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apoplast | - |
Solanum lycopersicum | 48046 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 L-ascorbate + O2 | Solanum lycopersicum | - |
4 monodehydroascorbate + 2 H2O | - |
? | |
4 L-ascorbate + O2 | Solanum lycopersicum West Virginia 106 | - |
4 monodehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | Q4VDN6 | - |
- |
Solanum lycopersicum West Virginia 106 | Q4VDN6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 L-ascorbate + O2 | - |
Solanum lycopersicum | 4 monodehydroascorbate + 2 H2O | - |
? | |
4 L-ascorbate + O2 | - |
Solanum lycopersicum West Virginia 106 | 4 monodehydroascorbate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ascorbate oxidase | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
malfunction | a diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit, overview. RNAi knockout plants show increased fruit yield under three conditions where assimilate became limiting for wild-type plants: when fruit trusses are left unpruned, when leaves are removed or when water supply is limited. Several alterations in the transgenic lines can contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. RNAi lines show altered sink strength | Solanum lycopersicum |
physiological function | ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool, effects of ascorbate oxidase on the apoplastic redox state, overview | Solanum lycopersicum |