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Literature summary for 1.10.3.3 extracted from
- Measuring nitric oxide in single neurons by capillary electrophoresis with laser-induced fluorescence: use of ascorbate oxidase in diaminofluorescein measurements (2006), Anal. Chem., 78, 1859-1865.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is required in NO measurements to eliminates the interference of ascorbic acid and/or dehydroascorbic acid in the assays, overview | Aplysia californica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbic acid + O2 | Aplysia californica | - |
2 L-dehydroascorbic acid + 2 H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aplysia californica | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mouth | homogenates of the mouth area | Aplysia californica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbic acid + O2 | - |
Aplysia californica | 2 L-dehydroascorbic acid + 2 H2O | - |
? | |
| additional information | ascorbic acid interferes with the realtime measurement of neuronal NO, an important gaseous neurotransmitter, by use of diaminofluoresceins, which act as receptors for NO, ascorbic acid reduces N2O3, the reaction intermediate of NO oxidation, while dehydroascorbic acid competes with NO for the receptors, while ascorbic acid inhibits the binding between NO and diaminofluoresceins, overview | Aplysia californica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ascorbate oxidase | - |
Aplysia californica |
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Release 2023.1 (January 2023)
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