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Literature summary for 1.10.3.3 extracted from

  • Sugino, M.; Kajita, S.; Banno, K.; Shirai, T.; Yamane, T.; Kato, M.; Kobayashi, T.; Tsukagoshi, N.
    Upward shift of the pH optimum of Acremonium ascorbate oxidase (2002), Biochim. Biophys. Acta, 1596, 36-46.
    View publication on PubMed
Search for more literature for 1.10.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type, Q183R, T527A, E177K, V185K, V188R, P190I, V193P, R194Q, Q382G, H383Y, V385K, R387A, A389N, L539E, H540L, M542L, V543H, W189H, W386H, V193G, V193F, V193H, V193K, V193E, V185K/V188R, V188R/P190I and P190I/V193P mutant enzyme in Aspergillus nidulans Acremonium

Protein Variants

Protein Variants Comment Organism
A389N slightly reduced thermostability Acremonium
E177K slightly reduced thermostability Acremonium
E540L slightly reduced thermostability Acremonium
H383Y slightly reduced thermostability Acremonium
L539E slightly increased thermostability Acremonium
M542L strongly reduced thermostability Acremonium
P190I reduced thermostability Acremonium
P190I/V193P pH optimum shifted to pH 4.5, slightly reduced thermostability Acremonium
Q183R similar properties as wild-type Acremonium
Q382G slightly higher thermostability Acremonium
R194Q similar properties as wild-type Acremonium
R387A slightly reduced thermostability Acremonium
T527A similar properties as wild-type Acremonium
V185K slightly reduced thermostability Acremonium
V185K/V188R similar properties as wild-type Acremonium
V188R/P190I no detectable activity Acremonium
V193E pH optimum shifted to pH 4.5, very thermolabile Acremonium
V193F slightly reduced thermostability Acremonium
V193G very thermolabile Acremonium
V193H slightly reduced thermostability Acremonium
V193K slightly reduced thermostability Acremonium
V193P pH optimum shifted to pH 5.0 Acremonium
V193T similar properties as wild-type Acremonium
V385K reduced thermostability Acremonium
V543H very thermolabile Acremonium
W189H no detectable activity Acremonium
W386H reduced thermostability Acremonium

Organism

Organism UniProt Comment Textmining
Acremonium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and various mutants Acremonium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Acremonium