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Literature summary for 1.10.3.3 extracted from

  • Bezerra Carvalho, L.; Lima, C.J.; Medeiros, P.H.
    Ascorbate oxidase from Cucurbita maxima (1981), Phytochemistry, 20, 2423-2424.
No PubMed abstract available
Search for more literature for 1.10.3.3

Inhibitors

Inhibitors Comment Organism Structure
azide 8 mM, 28% inhibition; weak inhibition Cucurbita maxima
Thiourea 300 mM, 55% inhibition Cucurbita maxima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.166
-
 L-ascorbate O2 consumption, polarographic method Cucurbita maxima
0.2
-
 L-ascorbate spectrophotometric assay Cucurbita maxima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Cucurbita maxima

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Cucurbita maxima
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-ascorbic acid + O2
-
 Cucurbita maxima 2 L-dehydroascorbic acid + 2 H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 30 min, stable Cucurbita maxima
100
-
 complete inactivation after 1 min Cucurbita maxima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 255% of the activity at pH 3.0 Cucurbita maxima