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Literature summary for 1.10.3.2 extracted from

  • Kim, C.; Lorenz, W.; Hoopes, J.; Dean, J.
    Oxidation of phenolate siderophores by the multicopper oxidase encoded by the Escherichia coli yacK gene (2001), J. Bacteriol., 183, 4866-4875 .
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 syringaldazine pH 5, 30°C Escherichia coli
0.04
-
 enterobactin pH 5, 30°C Escherichia coli
0.29
-
 2,3-Dihydroxybenzoic acid pH 5, 30°C Escherichia coli
0.43
-
 3,4-dihydroxybenzoic acid pH 5, 30°C Escherichia coli
0.69
-
 3-hydroxyanthranilic acid pH 5, 30°C Escherichia coli
2.12
-
 2,6-dimethoxyphenol pH 5, 30°C Escherichia coli
2.5
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) pH 5, 30°C Escherichia coli
3.2
-
 p-phenylenediamine pH 5, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains six copper atoms per polypeptide chain and displays optical and electron paramagnetic resonance spectra consistent with the presence of type 1, type 2, and type 3 copper centers. The addition of copper leads to immediate and reversible changes in the optical and electron paramagnetic resonance spectra of the protein, as well as decreased thermal stability of the enzyme and stimulates both the phenoloxidase and ferroxidase activities Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P36649 enzyme additionally displays copper oxidase activity, reaction of EC 1.16.3.4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
 substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), pH 5, 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Escherichia coli ?
-
 ?
2,3-dihydroxybenzoic acid + O2
-
 Escherichia coli ?
-
 ?
2,6-dimethoxyphenol + H2O
-
 Escherichia coli ?
-
 ?
3,4-dihydroxybenzoic acid + O2
-
 Escherichia coli ?
-
 ?
3-hydroxyanthranilic acid + O2
-
 Escherichia coli ?
-
 ?
enterobactin + O2
-
 Escherichia coli ?
-
 ?
additional information presence of Fe2+ reduces the apparent phenoloxidase activity, Cu(II) enhances this activity six- to sevenfold Escherichia coli ?
-
-
p-phenylenediamine + O2
-
 Escherichia coli ? + H2O
-
 ?
syringaldazine + O2
-
 Escherichia coli ?
-
 ?