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Literature summary for 1.10.3.2 extracted from

  • Sun, J.; Zheng, M.; Lu, Z.; Lu, F.; Zhang, C.
    Heterologous production of a temperature and pH-stable laccase from Bacillus vallismortis fmb-103 in Escherichia coli and its application (2017), Process Biochem., 55, 77-84 .
No PubMed abstract available
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 5 mM, 9-13% activation (depending on purification method) Bacillus vallismortis
Cu2+ 5 mM, 33-60% activation (depending on purification method) Bacillus vallismortis
Mg2+ 5 mM, 3-5% activation (depending on purification method) Bacillus vallismortis
Mn2+ 5 mM, 5-10% activation (depending on purification method) Bacillus vallismortis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Bacillus vallismortis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 84-87% inhibition (depending on purification method) Bacillus vallismortis
L-cysteine 0.1 mM, complete inhibition Bacillus vallismortis
NaCl 500 mM, 52-59% inhibition (depending on purification method) Bacillus vallismortis
SDS 1 mM, 37-41% inhibition (depending on purification method) Bacillus vallismortis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.358
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 45°C, pH 5.0 Bacillus vallismortis
0.379
-
 syringaldazine 45°C, pH 7.4 Bacillus vallismortis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 5 mM, 3-11% inhibition (depending on purification method) Bacillus vallismortis
K+ 5 mM, 6-12% inhibition (depending on purification method) Bacillus vallismortis
Zn2+ 5 mM, 80-87% inhibition (depending on purification method) Bacillus vallismortis

Organism

Organism UniProt Comment Textmining
Bacillus vallismortis
-
-
-
Bacillus vallismortis fmb-103
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus vallismortis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus vallismortis ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?
additional information Malachite green is efficiently degraded by recombinant laccase, especially in combination with mediators Bacillus vallismortis ?
-
 ?
additional information Malachite green is efficiently degraded by recombinant laccase, especially in combination with mediators Bacillus vallismortis fmb-103 ?
-
 ?
syringaldazine + O2
-
 Bacillus vallismortis ?
-
 ?
syringaldazine + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
84
-
-
 Bacillus vallismortis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 after incubation for 10 h the residual activity is greater than 50%. Activity increases by more than 120% after 1 h incubation Bacillus vallismortis
80
-
 ativity increases by nearly 90% after 1 h incubation Bacillus vallismortis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus vallismortis
7.4
-
 substrate: syringaldazine Bacillus vallismortis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 10 days, 75-80% loss of activity (depending on purification method) Bacillus vallismortis
7
-
 10 days, 30-40% loss of activity (depending on purification method) Bacillus vallismortis
8
-
 10 days, 10-20% loss of activity (depending on purification method) Bacillus vallismortis