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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of the laccase involved in dye decolorization by the white-rot fungus Marasmius scorodonius (2017), J. Microbiol. Biotechnol., 27, 1120-1127 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment, thermostable and acidophilic laccase that can efficiently decolorize several synthetic dyes without addition of an expensive redox mediator | Mycetinis scorodonius |
| environmental protection | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment, thermostable and acidophilic laccase that can efficiently decolorize several synthetic dyes without addition of an expensive redox mediator | Mycetinis scorodonius |
| industry | the enzyme can be usedc in industrial bleaching processes, thermostable and acidophilic laccase that can efficiently decolorize several synthetic dyes without an expensive redox mediator | Mycetinis scorodonius |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme was stable in the presence of some metal ions such as Ca2+, Cu2+, Ni2+, Mg2+, Mn2+, Ba2+, Co2+, and Zn2+ at 1 mM | Mycetinis scorodonius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 97% inhibition | Mycetinis scorodonius |  |
| Ba2+ | 10 mM, 59% inhibition | Mycetinis scorodonius | |
| Ca2+ | 10 mM, 80% inhibition | Mycetinis scorodonius | |
| Co2+ | 10 mM, 55% inhibition | Mycetinis scorodonius | |
| Cu2+ | 10 mM, 61% inhibition | Mycetinis scorodonius | |
| dithiothreitol | 1 mM, 100% inhibition | Mycetinis scorodonius | |
| EDTA | 10 mM, 6% inhibition | Mycetinis scorodonius | |
| Fe2+ | 1 mM, complete inhibition | Mycetinis scorodonius | |
| L-cysteine | 1 mM, 100% inhibition | Mycetinis scorodonius | |
| Mg2+ | 10 mM, 66% inhibition | Mycetinis scorodonius | |
| Mn2+ | 10 mM, 72% inhibition | Mycetinis scorodonius | |
| NaN3 | 1 mM, 98% inhibition | Mycetinis scorodonius | |
| Ni2+ | 10 mM, 68% inhibition | Mycetinis scorodonius | |
| p-coumaric acid | 1 mM, 48% inhibition | Mycetinis scorodonius | |
| SDS | 1 mM, 43% inhibition | Mycetinis scorodonius | |
| tropolone | 1 mM, 78% inhibition | Mycetinis scorodonius | |
| Zn2+ | 10 mM, 88% inhibition | Mycetinis scorodonius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.4, 70°C | Mycetinis scorodonius | |
| 0.602 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
| 2.772 | - |
guaiacol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Mycetinis scorodonius | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein | Mycetinis scorodonius | |
| Fe2+ | the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein | Mycetinis scorodonius | |
| Zn2+ | the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein | Mycetinis scorodonius | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycetinis scorodonius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycetinis scorodonius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Mycetinis scorodonius | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Mycetinis scorodonius | ? | - |
? | |
| guaiacol + O2 | - |
Mycetinis scorodonius | ? | - |
? | |
| additional information | the enzyme decolorizes Congo red, Malachite green, Crystal violet, and Methylene green dyes at various decolorization rates of 63-90% without the addition of an expensive mediator | Mycetinis scorodonius | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Mycetinis scorodonius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Mycetinis scorodonius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 90 | 55°C: about 50% of maximal activity, 90°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Mycetinis scorodonius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
100 mim, 20% loss of activity | Mycetinis scorodonius |
| 75 | - |
40 min, enzyme hardly retains any activity | Mycetinis scorodonius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 68 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
| 72 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.4, 70°C | Mycetinis scorodonius | |
| 226 | - |
guaiacol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.4 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Mycetinis scorodonius |
| 4 | - |
substrate: guaiacol | Mycetinis scorodonius |
| 4.6 | - |
substrate: 2,6-dimethoxyphenol | Mycetinis scorodonius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | 4.2 | pH 2.2: about 40% of maximal activity, pH 4.2: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Mycetinis scorodonius |
| 2.2 | 4.2 | pH 2.2: about 40% of maximal activity, pH 4.2: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol | Mycetinis scorodonius |
| 3.5 | 5.5 | pH 3.5: about 70% of maximal activity, pH 5.5: about 60% of maximal activity, substrate: guaiacol | Mycetinis scorodonius |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 81 | - |
guaiacol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
| 113 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Mycetinis scorodonius | |
| 2609 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.4, 70°C | Mycetinis scorodonius | |
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Release 2023.1 (January 2023)
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