Application | Comment | Organism |
---|---|---|
degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme is effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorizationefficiencies of 50%-85% | Cerrena unicolor |
environmental protection | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme is effective in the decolorization of bromothymol blue, evans blue, methyl orange, and malachite green with decolorizationefficiencies of 50%-85% | Cerrena unicolor |
Cloned (Comment) | Organism |
---|---|
- |
Cerrena unicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.25 mM, 8% inhibition. 10 mM, 17% inhibition | Cerrena unicolor | |
Cd2+ | 1.25 mM, 6% inhibition. 10 mM, 20% inhibition | Cerrena unicolor | |
Co2+ | 1.25 mM, 3% inhibition. 10 mM, 17% inhibition | Cerrena unicolor | |
Cu2+ | 1.25 mM, 9% inhibition. 10 mM, 19% inhibition | Cerrena unicolor | |
EDTA | 10 mM, 10% inhibition | Cerrena unicolor | |
Fe2+ | 1.25 mM, 31% inhibition. 10 mM, 61% inhibition | Cerrena unicolor | |
Fe3+ | 1.25 mM, 46% inhibition. 10 mM, 93% inhibition | Cerrena unicolor | |
K+ | 10 mM, 4% inhibition | Cerrena unicolor | |
Mg2+ | 10 mM, 8% inhibition | Cerrena unicolor | |
Na+ | 1.25 mM, 4% inhibition. 10 mM, 20% inhibition | Cerrena unicolor | |
Zn2+ | 10 mM, 13% inhibition | Cerrena unicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.303 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 25°C | Cerrena unicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cerrena unicolor | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 1.25 mM, 1.08fold activation | Cerrena unicolor | |
Mn2+ | 1.25-10 mM, slightly enhance the laccase activity of 3.810.5% | Cerrena unicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63200 | - |
- |
Cerrena unicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena unicolor | - |
- |
- |
Cerrena unicolor GSM-01 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cerrena unicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Cerrena unicolor | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Cerrena unicolor GSM-01 | ? | - |
? | |
catechol + O2 | 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor | ? | - |
? | |
catechol + O2 | 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor GSM-01 | ? | - |
? | |
guaiacol + O2 | 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor | ? | - |
? | |
guaiacol + O2 | 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor GSM-01 | ? | - |
? | |
o-toluidine + O2 | 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor | ? | - |
? | |
o-toluidine + O2 | 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor GSM-01 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63200,SDS-PAGE | Cerrena unicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 55% of maximal activity, 65°C: about 55% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
286.5 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 25°C | Cerrena unicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.6 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 4.3 | pH 2.2: about 90% of maximal activity, pH 4.3: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena unicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
946 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.0, 25°C | Cerrena unicolor |