Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Jaiswal, N.; Pandey, V.P.; Dwivedi, U.N.
    Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography (2015), Int. J. Biol. Macromol., 72, 326-332 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM Carica papaya

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM Carica papaya
EDTA
-
Carica papaya
Hg2+ concentration-dependent inhibition up to 1 mM Carica papaya
Na+ exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity Carica papaya
SDS
-
Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya
1.48
-
catechol pH 7.5, 37°C Carica papaya
11.33
-
hydroquinone pH 7.5, 37°C Carica papaya

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
Cd2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
Co2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
Cu2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
Fe2+ activates laccase in aconcentration-dependent manner up to 1 mM Carica papaya
Mn2+ activates laccase in a concentration-dependent manner (0.1-10 mM) Carica papaya
Na+ exhibits activation of laccase up to 1 mM beyond which it exhibited inhibition of laccase activity Carica papaya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
260000
-
gel filtration Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two step procedure: heat treatment (at 70°C) and Con-A affinity chromatography Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Carica papaya
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.3
-
pH 7.5, 37°C Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Carica papaya ?
-
?
2-benzoquinone + O2
-
Carica papaya ?
-
?
catechol + O2
-
Carica papaya ?
-
?
hydroquinone + O2
-
Carica papaya ?
-
?

Subunits

Subunits Comment Organism
hexamer 2 * 52000 + 2 * 45000 + 1 * 38000 + 1 * 25000, SDS-PAGE Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Carica papaya

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
1 h, 25% loss of activity Carica papaya
60
-
1 h, 18% loss of activity Carica papaya
70
-
1 h, 47% loss of activity Carica papaya
80
-
1 h, complete loss of activity Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00403
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya
0.0165
-
catechol pH 7.5, 37°C Carica papaya
0.051
-
hydroquinone pH 7.5, 37°C Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
non-phenolic substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Carica papaya
8
-
phenolic substrate: catechol Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 65% of maximal activity, pH 9.0: 72% of maximal activity Carica papaya

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 24 h, 37°C, the enzyme retains more than 75% of initial activity Carica papaya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0045
-
hydroquinone pH 7.5, 37°C Carica papaya
0.011
-
catechol pH 7.5, 37°C Carica papaya
0.1
-
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 37°C Carica papaya