Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM | Carica papaya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM | Carica papaya | |
EDTA | - |
Carica papaya | |
Hg2+ | concentration-dependent inhibition up to 1 mM | Carica papaya | |
Na+ | exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity | Carica papaya | |
SDS | - |
Carica papaya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya | |
1.48 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
11.33 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
Cd2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
Co2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
Cu2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
Fe2+ | activates laccase in aconcentration-dependent manner up to 1 mM | Carica papaya | |
Mn2+ | activates laccase in a concentration-dependent manner (0.1-10 mM) | Carica papaya | |
Na+ | exhibits activation of laccase up to 1 mM beyond which it exhibited inhibition of laccase activity | Carica papaya |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
260000 | - |
gel filtration | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two step procedure: heat treatment (at 70°C) and Con-A affinity chromatography | Carica papaya |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Carica papaya | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.3 | - |
pH 7.5, 37°C | Carica papaya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Carica papaya | ? | - |
? | |
2-benzoquinone + O2 | - |
Carica papaya | ? | - |
? | |
catechol + O2 | - |
Carica papaya | ? | - |
? | |
hydroquinone + O2 | - |
Carica papaya | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 2 * 52000 + 2 * 45000 + 1 * 38000 + 1 * 25000, SDS-PAGE | Carica papaya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Carica papaya |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity | Carica papaya |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 25% loss of activity | Carica papaya |
60 | - |
1 h, 18% loss of activity | Carica papaya |
70 | - |
1 h, 47% loss of activity | Carica papaya |
80 | - |
1 h, complete loss of activity | Carica papaya |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00403 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya | |
0.0165 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
0.051 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
non-phenolic substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Carica papaya |
8 | - |
phenolic substrate: catechol | Carica papaya |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: about 65% of maximal activity, pH 9.0: 72% of maximal activity | Carica papaya |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 24 h, 37°C, the enzyme retains more than 75% of initial activity | Carica papaya |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
hydroquinone | pH 7.5, 37°C | Carica papaya | |
0.011 | - |
catechol | pH 7.5, 37°C | Carica papaya | |
0.1 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 7.5, 37°C | Carica papaya |