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Literature summary for 1.10.3.2 extracted from
- Isolation, one-step affinity purification, and characterization of a polyextremotolerant laccase from the halophilic bacterium Aquisalibacillus elongatus and its application in the delignification of sugar beet pulp (2017), Biores. Technol., 230, 67-75 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | delignification of sugar beet pulp. Delignification pretreatment is the most challenging and critical step in the recycling process of lignocellulosic materials as the most abundant natural resources on the earth and has a large impact on cellulose extraction, cellulose digestibility, and downstream costs. Amongst the various delignification strategies, the enzymatic method has been demonstrated as a promising eco-friendly process with no inhibitor generation, although it is not highly efficient | Aquisalibacillus elongatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0082 | - |
2,6-dimethoxyphenol | pH 8.0, 40°C | Aquisalibacillus elongatus |  |
| 0.0145 | - |
pyrogallol | pH 8.0, 40°C | Aquisalibacillus elongatus | |
| 0.0161 | - |
syringaldazine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0161 | - |
2,5-xylidine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0251 | - |
Tannic acid | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0392 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6.0, 40°C | Aquisalibacillus elongatus | |
| 0.0514 | - |
gallic acid | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0518 | - |
L-tyrosine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0686 | - |
hydroquinone | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.0773 | - |
catechol | pH 9.0, 40°C | Aquisalibacillus elongatus | |
| 0.102 | - |
L-Dopa | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 0.367 | - |
guaiacol | pH 8.0, 40°C | Aquisalibacillus elongatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aquisalibacillus elongatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
nondenaturing PAGE | Aquisalibacillus elongatus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| diethylene triamine | amongst 42 solvents tested, significant deactivation is observed only in the presence of diethylene triamine (less than 50% of activity retains after 6 h incubation), which is probably due to the stripping-off of the crucial bound-water monolayer from the enzyme molecule essential for its activity | Aquisalibacillus elongatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquisalibacillus elongatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content: 8.1% | Aquisalibacillus elongatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| one-step purification | Aquisalibacillus elongatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 498.9 | - |
pH 8.0, 40°C, substrate: 2,6-dimethoxyphenol | Aquisalibacillus elongatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| 2,5-xylidine + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| catechol + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| gallic acid + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| guaiacol + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| hydroquinone + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| L-DOPA + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| L-tyrosine + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| pyrogallol + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| syringaldazine + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
| tannic acid + O2 | - |
Aquisalibacillus elongatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 75000, nondenaturing PAGE | Aquisalibacillus elongatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
substrates: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine or 2,6-dimethoxyphenol | Aquisalibacillus elongatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | stable | Aquisalibacillus elongatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56.3 | - |
guaiacol | pH 8.0, 40°C | Aquisalibacillus elongatus | |
| 284.4 | - |
2,5-xylidine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 318.8 | - |
L-Dopa | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 412.5 | - |
catechol | pH 9.0, 40°C | Aquisalibacillus elongatus | |
| 443.8 | - |
hydroquinone | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 462.5 | - |
L-tyrosine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 675 | - |
gallic acid | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 918.8 | - |
syringaldazine | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 1009.4 | - |
2,6-dimethoxyphenol | pH 8.0, 40°C | Aquisalibacillus elongatus | |
| 1231.3 | - |
pyrogallol | pH 8.0, 40°C | Aquisalibacillus elongatus | |
| 1471.9 | - |
Tannic acid | pH 7.0, 40°C | Aquisalibacillus elongatus | |
| 2150 | - |
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6.0, 40°C | Aquisalibacillus elongatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Aquisalibacillus elongatus |
| 7 | - |
substrate: syringaldazine | Aquisalibacillus elongatus |
| 8 | - |
substrate: 2,6-dimethoxyphenol | Aquisalibacillus elongatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | stable at pH 5.0-10.0. Activity is drastically reduced at pH values lower than 5.0 after 6 h incubation | Aquisalibacillus elongatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aquisalibacillus elongatus | isoelectric focusing | - |
6.8 |
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