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Literature summary for 1.10.3.2 extracted from
- Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes (2015), Biochimie, 112, 151-159 .
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. The enzyme can also be considered as a candidate for treating industrial effluent containing malachite green | Streptomyces viridochromogenes |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced in Escherichia coli | Streptomyces viridochromogenes |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapour-diffusion method, X-ray quality crystals are generated and the structure is solved to a resolution of 2.4 A | Streptomyces viridochromogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Streptomyces viridochromogenes |  |
| additional information | the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF | Streptomyces viridochromogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
guaiacol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 0.38 | - |
2,6-dimethoxyphenol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 4.5 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 9.9 | - |
K4[Fe(CN)6] | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | the recombinant protein is found in the cytoplasmic fraction | Streptomyces viridochromogenes | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | copper-containing enzyme. Contains 4 atoms per molecule of the enzyme | Streptomyces viridochromogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | 90000 | gel filtration | Streptomyces viridochromogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridochromogenes | J9S5G3 | - |
- |
| Streptomyces viridochromogenes VKM Ac-629 | J9S5G3 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity | Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Streptomyces viridochromogenes | ? | - |
? | |
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Streptomyces viridochromogenes VKM Ac-629 | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Streptomyces viridochromogenes | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Streptomyces viridochromogenes VKM Ac-629 | ? | - |
? | |
| guaiacol + O2 | - |
Streptomyces viridochromogenes | ? | - |
? | |
| guaiacol + O2 | - |
Streptomyces viridochromogenes VKM Ac-629 | ? | - |
? | |
| K4[Fe(CN)6] + O2 | - |
Streptomyces viridochromogenes | ? | - |
? | |
| additional information | phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity | Streptomyces viridochromogenes | ? | - |
? | |
| additional information | phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity | Streptomyces viridochromogenes VKM Ac-629 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 33900, calculated from sequence | Streptomyces viridochromogenes |
| homotrimer | 3 * 39000, SDS-PAGE after boiling | Streptomyces viridochromogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
K4[Fe(CN)6] | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 1.9 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 8 | - |
guaiacol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 34.4 | - |
2,6-dimethoxyphenol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
below, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Streptomyces viridochromogenes |
| 8.5 | - |
substrate: 2,6-dimethoxyphenol | Streptomyces viridochromogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH | Streptomyces viridochromogenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
20°C, 2 days, the enzyme retains about 30% residual activity | Streptomyces viridochromogenes |
| 3 | - |
20°C, 2 days, the enzyme retains about 42% residual activity | Streptomyces viridochromogenes |
| 4 | - |
20°C, 2 days, the enzyme retains about 65-60% residual activity | Streptomyces viridochromogenes |
| 5 | - |
20°C, 2 days, the enzyme retains about 65-60% residual activity | Streptomyces viridochromogenes |
| 6 | 10 | 20°C, 2 days, the enzyme retains about 70% residual activity | Streptomyces viridochromogenes |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 8 | - |
pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | EDTA | |
| 14 | - |
pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | EDTA | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
K4[Fe(CN)6] | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 0.42 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 26.6 | - |
guaiacol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
| 90.5 | - |
2,6-dimethoxyphenol | pH 8.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
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