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Literature summary for 1.10.3.2 extracted from
- Overexpression and characterization of laccase from Trametes versicolor in Pichia pastoris (2014), Appl. Biochem. Microbiol., 50, 140-147 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Pichia pastoris | Trametes versicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Trametes versicolor |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.5, 55°C | Trametes versicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | significantly enhances activity | Trametes versicolor | |
| Cu2+ | significantly enhances activity | Trametes versicolor | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes versicolor | I6QS85 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Trametes versicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.8 | - |
pH 4.5, 55°C, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes versicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Trametes versicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LccA | - |
Trametes versicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes versicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | 30°C: about 70% of maximal activity, 80°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes versicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 2 h, stable | Trametes versicolor |
| 60 | - |
1 h, residual activity is more that 50% | Trametes versicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes versicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 7 | 6 h, 25°C, in the absence of the substrate, recombinant laccase is stable | Trametes versicolor |
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