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Literature summary for 1.10.3.2 extracted from

  • Chaurasia, P.K.; Yadav, A.; Yadav, S.S.; Yadava, S.
    Purification and characterization of laccase secreted by Phellinus linteus MTCC-1175 and its role in the selective oxidation of aromatic methyl group (2014), Appl. Biochem. Microbiol., 49, 592-599 .
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Tropicoporus linteus
0.092
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH and temperature not specified in the publication Tropicoporus linteus
0.16
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Tropicoporus linteus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Tropicoporus linteus
-
-

Organism

Organism UniProt Comment Textmining
Tropicoporus linteus
-
-
-
Tropicoporus linteus MTCC-1175
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tropicoporus linteus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Tropicoporus linteus ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Tropicoporus linteus MTCC-1175 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Tropicoporus linteus ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Tropicoporus linteus MTCC-1175 ?
-
 ?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
-
 Tropicoporus linteus ?
-
 ?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
-
 Tropicoporus linteus MTCC-1175 ?
-
 ?
additional information in the presence of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the mediator, the enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively Tropicoporus linteus ?
-
 ?
additional information in the presence of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the mediator, the enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively Tropicoporus linteus MTCC-1175 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Tropicoporus linteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the activation energy for thermal denaturation of the enzyme is 38.20 kJ/mole/K Tropicoporus linteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.85
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Tropicoporus linteus
6.85
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH and temperature not specified in the publication Tropicoporus linteus
6.85
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Tropicoporus linteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 the enzyme iss the most stable at pH 5.0 after 1 h reaction Tropicoporus linteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42.8
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Tropicoporus linteus
74.4
-
 4-hydroxy-3,5-dimethoxybenzaldehyde azine pH and temperature not specified in the publication Tropicoporus linteus
163.1
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Tropicoporus linteus