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Literature summary for 1.10.3.2 extracted from

  • Guan, Z.; Zhang, N.; Song, C.; Zhou, W.; Zhou, L.; Zhao, H.; Xu, C.; Cai, Y.; Liao, X.
    Molecular cloning, characterization, and dye-decolorizing ability of a temperature- and pH-stable laccase from Bacillus subtilis X1 (2014), Appl. Biochem. Biotechnol., 172, 1147-1157 .
    View publication on PubMed

Application

Application Comment Organism
degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 Bacillus subtilis X1
environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 Bacillus subtilis X1

Cloned(Commentary)

Cloned (Comment) Organism
efficiently expressed in Escherichia coli in a biologically active form Bacillus subtilis X1

Organism

Organism UniProt Comment Textmining
Bacillus subtilis X1 M9Y1F2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis X1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus subtilis X1 ?
-
?
2,6-dimethoxyphenol + O2
-
Bacillus subtilis X1 ?
-
?
additional information two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 Bacillus subtilis X1 ?
-
?
syringaldazine + O2
-
Bacillus subtilis X1 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus subtilis X1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 30°C: about 50% of maximal activity, 80°C: about 45% of maximal activity Bacillus subtilis X1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 h, no loss of activity Bacillus subtilis X1
60
-
10 h, no loss of activity Bacillus subtilis X1
70
-
half-life: 6 h Bacillus subtilis X1
80
-
half-life: 2.5 h, 21 % of the initial activity is detected after 10 h Bacillus subtilis X1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus subtilis X1
6.9
-
substrate: syringaldazine Bacillus subtilis X1
7.3
-
substrate: 2,6-dimethoxyphenol Bacillus subtilis X1

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
unstable at, 23 and 15% of the original activity remains after incubation for 1 and 3 days respectively Bacillus subtilis X1
7
-
37°C, no loss of laccase activity after 10 days of incubation Bacillus subtilis X1
9
-
37°C, no loss of laccase activity after 10 days of incubation Bacillus subtilis X1