Cloned (Comment) | Organism |
---|---|
gene lac1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Pichia pastoris and Escherichia coli strain DH5aalpha | Ganoderma fornicatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA | Ganoderma fornicatum | |
Sodium azide | strong inhibition | Ganoderma fornicatum | |
thioglycolic acid | strong inhibition | Ganoderma fornicatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.104 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
0.277 | - |
2,6-dimethoxyphenol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
1.263 | - |
guaiacol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54100 | - |
x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation | Ganoderma fornicatum |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | rLac1 retains less than 20% activity in 50% v/v acetone after a 3 h incubation at 25°C | Ganoderma fornicatum |
acetonitril | rLac1 retains less than 20% activity in 50% v/v acetonitril after a 3 h incubation at 25°C | Ganoderma fornicatum |
dimethylformamide | rLac1 retains more than 80% activity in 50% v/v dimethylformamide after a 3 h incubation at 25°C | Ganoderma fornicatum |
DMSO | rLac1 retains more than 80% activity in 50% v/v DMSO after a 3 h incubation at 25°C | Ganoderma fornicatum |
Ethanol | rLac1 retains more than 80% activity in 50% v/v ethanol after a 3 h incubation at 25°C | Ganoderma fornicatum |
Methanol | rLac1 retains more than 80% activity in 50% v/v methanol after a 3 h incubation at 25°C | Ganoderma fornicatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ganoderma fornicatum | B5G555 | gene lac1 | - |
Ganoderma fornicatum 0814 | B5G555 | gene lac1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycoslation with endoglycosylase H | Ganoderma fornicatum |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 3.8fold by ulrafiltration, anion exchange chromatography, again ultrafiltration, and gel filtration | Ganoderma fornicatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
59.27 | - |
purified recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Ganoderma fornicatum | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Ganoderma fornicatum 0814 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Ganoderma fornicatum | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Ganoderma fornicatum 0814 | ? | - |
? | |
guaiacol + O2 | - |
Ganoderma fornicatum | ? | - |
? | |
guaiacol + O2 | - |
Ganoderma fornicatum 0814 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x + 63700, glycosylated recombinant enzyme, SDS-PAGE, x * 54100, about, sequence calculation | Ganoderma fornicatum |
Synonyms | Comment | Organism |
---|---|---|
LAC1 | - |
Ganoderma fornicatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Ganoderma fornicatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | activity range | Ganoderma fornicatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | recombinant enzyme, pH 5.0 for 9 h, stable | Ganoderma fornicatum |
70 | - |
recombinant enzyme, pH 5.0 for 9 h, loss of 80% activity | Ganoderma fornicatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
guaiacol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
38.5 | - |
2,6-dimethoxyphenol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
65.6 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Ganoderma fornicatum |
3 | - |
with substrates 2,6-dimethoxyphenol and guaiacol | Ganoderma fornicatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5 | activity range | Ganoderma fornicatum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 10 | recombinant enzyme, rLac1 retains more than 80% of its activity in solutions within this range after a 24 h incubation at 25°C | Ganoderma fornicatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ganoderma fornicatum | sequence calculation | - |
5.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
guaiacol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
140 | - |
2,6-dimethoxyphenol | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum | |
630 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | recombinant enzyme, pH 3.0, 30°C | Ganoderma fornicatum |