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Literature summary for 1.10.3.2 extracted from

  • Lin, Y.; Zhang, Z.; Tian, Y.; Zhao, W.; Zhu, B.; Xu, Z.; Peng, R.; Yao, Q.
    Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes (2013), Mol. Biol. Rep., 40, 1487-1494.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene CcLCC5I, expresssion in Pichia pastoris GS115 Coprinopsis cinerea

General Stability

General Stability Organism
the activity of CcLCC5I is stable after incubation for 16 h at 4°C in 0.2 M citrate-phosphate buffer at weakly alkaline conditions, the minimal effect on enzyme activity is at pH 8.6 Coprinopsis cinerea

Inhibitors

Inhibitors Comment Organism Structure
Al3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Ba2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Ca2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Cr3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
dithiothreitol
-
 Coprinopsis cinerea
Fe2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Fe3+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
K+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
L-cysteine
-
 Coprinopsis cinerea
Li+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Mg2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
Mn2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
additional information except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity Coprinopsis cinerea
Na+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea
NaN3
-
 Coprinopsis cinerea
sodium dodecylsulfate
-
 Coprinopsis cinerea
sodium sulfite
-
 Coprinopsis cinerea
sodium thiosulfate
-
 Coprinopsis cinerea
Zn2+ activating at 1 mM, inhibitory at 10 mM Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.086
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea
17.6
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea

Metals/Ions

Metals/Ions Comment Organism Structure
additional information except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity Coprinopsis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56800
-
 x * 56800, SDS-PAGE Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea A8N3P5 gene CcLCC5I
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinopsis cinerea ?
-
 ?
guaiacol + O2
-
 Coprinopsis cinerea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56800, SDS-PAGE Coprinopsis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Coprinopsis cinerea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 optimum temperature of CcLCC5I is 55°C, the activity of laccase is high from 30 to 50°C, when the temperature is over 55°C, the activity declines more, when the temperature reaches 70°C, the relative activity of Lcc is only 23%, profile, overview Coprinopsis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 recombinant enzyme, stable up to Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.211
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea
4.22
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Coprinopsis cinerea

pH Range

pH Minimum pH Maximum Comment Organism
2 7 activity range, profile, overview Coprinopsis cinerea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
 guaiacol pH 3.0, 30°C Coprinopsis cinerea
49.1
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 30°C Coprinopsis cinerea