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Literature summary for 1.10.3.2 extracted from
- Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum (2011), BMC Biotechnol., 11, 9-9.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product | Bacillus pumilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CotA-type laccase, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 | Bacillus pumilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics do not fit a Michaelis-Menten equation | Bacillus pumilus | |
| 0.08 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 22°C, recombinant enzyme | Bacillus pumilus |  |
| 0.68 | - |
2,6-dimethoxyphenol | pH 7.0, 22°C, recombinant enzyme | Bacillus pumilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the CotA-type laccase is associated with 4 Cu2+ ions, content determined by inductive coupled plasma optical emission spectroscopy | Bacillus pumilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, recombinant CotA-type laccase, SDS-PAGE | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
gene cotA | - |
| Bacillus pumilus DSM 27 | - |
gene cotA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CotA-type laccase from Escherichia coli strain JM109 by anion exchange chromatography, ultrafiltration, and gel filtration | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| (2Z,2'Z)-2,2'-(1Z,2Z)-hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 1-(2,4,5-trihydroxyphenyl)butan-1-one + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 1-(4-hydroxy-3,5-dimethoxyphenyl)ethanone + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
| 3,4,5-trihydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 3,4,5-trihydroxybenzoic acid + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
| 3,4-dihydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 3,4-dihydroxybenzoic acid + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
| 3-amino-4-hydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 3-hydroxy-L-tyrosine + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-(2-aminoethyl)benzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-(hydroxymethyl)-2-methoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-amino-3-hydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzaldehyde + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 4-methylbenzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 5-(hydroxymethyl)-2-methoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| benzene-1,2,3-triol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| benzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus pumilus | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, recombinant CotA-type laccase, SDS-PAGE | Bacillus pumilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CotA-type laccase | - |
Bacillus pumilus |
| spore coat protein A | - |
Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus pumilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 75 | activity range | Bacillus pumilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.9 | - |
syringaldazine | pH 6.5, 22°C, recombinant enzyme, with 0.8% v/v DMSO | Bacillus pumilus | |
| 11 | - |
2,6-dimethoxyphenol | pH 7.0, 22°C, recombinant enzyme | Bacillus pumilus | |
| 66 | - |
syringaldazine | pH 7.0, 22°C, recombinant enzyme, with 25% v/v DMSO | Bacillus pumilus | |
| 291 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 22°C, recombinant enzyme | Bacillus pumilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | broad optimum, dependent on the substrate | Bacillus pumilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | activity range | Bacillus pumilus |
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