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Literature summary for 1.10.3.2 extracted from

  • Kaushik, G.; Thakur, I.
    Purification, characterization and usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes (2013), Appl. Biochem. Microbiol., 49, 352-359.
No PubMed abstract available

Application

Application Comment Organism
additional information usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes, overview Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) shows substrate inhibition at high concentrations above 6 mM Bacillus sp. (in: Bacteria)
acetonitrile completely inhibits enzyme activity Bacillus sp. (in: Bacteria)
EDTA 28% inhibition at 40 mM Bacillus sp. (in: Bacteria)
methanol completely inhibits enzyme activity Bacillus sp. (in: Bacteria)
Sodium azide completely inhibits enzyme activity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
isolated from distillery effluent and sludge
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 6.6fold from culture supernatant by anion exchange chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
952
-
puriifed native extracellular enzyme, pH 3.0, 22°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus sp. (in: Bacteria) ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 activity range Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
purified native enzyme, half-life is 116 min Bacillus sp. (in: Bacteria)
35
-
purified native enzyme, half-life is 104 min Bacillus sp. (in: Bacteria)
45
-
purified native enzyme, half-life is 67 min Bacillus sp. (in: Bacteria)
55
-
purified native enzyme, half-life is 47 min Bacillus sp. (in: Bacteria)
65
-
purified native enzyme, half-life is 33 min Bacillus sp. (in: Bacteria)
75
-
purified native enzyme, half-life is 23 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Bacillus sp. (in: Bacteria)