Application | Comment | Organism |
---|---|---|
additional information | usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes, overview | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | shows substrate inhibition at high concentrations above 6 mM | Bacillus sp. (in: Bacteria) | |
acetonitrile | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) | |
EDTA | 28% inhibition at 40 mM | Bacillus sp. (in: Bacteria) | |
methanol | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) | |
Sodium azide | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
isolated from distillery effluent and sludge | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 6.6fold from culture supernatant by anion exchange chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
952 | - |
puriifed native extracellular enzyme, pH 3.0, 22°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | activity range | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
purified native enzyme, half-life is 116 min | Bacillus sp. (in: Bacteria) |
35 | - |
purified native enzyme, half-life is 104 min | Bacillus sp. (in: Bacteria) |
45 | - |
purified native enzyme, half-life is 67 min | Bacillus sp. (in: Bacteria) |
55 | - |
purified native enzyme, half-life is 47 min | Bacillus sp. (in: Bacteria) |
65 | - |
purified native enzyme, half-life is 33 min | Bacillus sp. (in: Bacteria) |
75 | - |
purified native enzyme, half-life is 23 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Bacillus sp. (in: Bacteria) |