Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Kumar, G.N.; Srikumar, K.
    Characterization of xerophytic thermophilic laccase exhibiting metal ion-dependent dye decolorization potential (2012), Appl. Biochem. Biotechnol., 167, 662-676.
    View publication on PubMed

General Stability

General Stability Organism
the purified native enzyme is stable in 4 M urea Deamia testudo
the purified native enzyme is stable in 4 M urea Opuntia vulgaris

Inhibitors

Inhibitors Comment Organism Structure
cysteine-HCl complete inhibition at 0.1 mM Deamia testudo
cysteine-HCl complete inhibition at 0.1 mM Opuntia vulgaris
EDTA complete inhibition at 1 mM Deamia testudo
EDTA complete inhibition at 1 mM Opuntia vulgaris
Fe2+ complete inhibition at 10 mM Deamia testudo
Fe2+ complete inhibition at 10 mM Opuntia vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates 40% at 1 mM Deamia testudo
Cu2+ activates 40% at 1 mM Opuntia vulgaris
Mn2+ activates 40% at 1 mM Deamia testudo
Mn2+ activates 40% at 1 mM Opuntia vulgaris

Organism

Organism UniProt Comment Textmining
Deamia testudo
-
different isozymes
-
Opuntia vulgaris
-
different isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from cladodes to homogeneity by anion exchange chromatography and gel filtration Deamia testudo
native enzyme from cladodes to homogeneity by anion exchange chromatography and gel filtration Opuntia vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
cladode
-
Deamia testudo
-
cladode
-
Opuntia vulgaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 2,6-dimethoxyphenol + O2
-
Deamia testudo ?
-
?
4 2,6-dimethoxyphenol + O2
-
Opuntia vulgaris ?
-
?
additional information in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview Deamia testudo ?
-
?
additional information in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview Opuntia vulgaris ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 90 assay at Deamia testudo
70 80 assay at Opuntia vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 activity range Deamia testudo
30 100 activity range Opuntia vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
assay at Deamia testudo
10
-
assay at Opuntia vulgaris