General Stability | Organism |
---|---|
the purified native enzyme is stable in 4 M urea | Deamia testudo |
the purified native enzyme is stable in 4 M urea | Opuntia vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine-HCl | complete inhibition at 0.1 mM | Deamia testudo | |
cysteine-HCl | complete inhibition at 0.1 mM | Opuntia vulgaris | |
EDTA | complete inhibition at 1 mM | Deamia testudo | |
EDTA | complete inhibition at 1 mM | Opuntia vulgaris | |
Fe2+ | complete inhibition at 10 mM | Deamia testudo | |
Fe2+ | complete inhibition at 10 mM | Opuntia vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates 40% at 1 mM | Deamia testudo | |
Cu2+ | activates 40% at 1 mM | Opuntia vulgaris | |
Mn2+ | activates 40% at 1 mM | Deamia testudo | |
Mn2+ | activates 40% at 1 mM | Opuntia vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deamia testudo | - |
different isozymes | - |
Opuntia vulgaris | - |
different isozymes | - |
Purification (Comment) | Organism |
---|---|
native enzyme from cladodes to homogeneity by anion exchange chromatography and gel filtration | Deamia testudo |
native enzyme from cladodes to homogeneity by anion exchange chromatography and gel filtration | Opuntia vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cladode | - |
Deamia testudo | - |
cladode | - |
Opuntia vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 2,6-dimethoxyphenol + O2 | - |
Deamia testudo | ? | - |
? | |
4 2,6-dimethoxyphenol + O2 | - |
Opuntia vulgaris | ? | - |
? | |
additional information | in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview | Deamia testudo | ? | - |
? | |
additional information | in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview | Opuntia vulgaris | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | assay at | Deamia testudo |
70 | 80 | assay at | Opuntia vulgaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | activity range | Deamia testudo |
30 | 100 | activity range | Opuntia vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at | Deamia testudo |
10 | - |
assay at | Opuntia vulgaris |