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Literature summary for 1.10.3.2 extracted from

  • Mohammadian, M.; Fathi-Roudsari, M.; Mollania, N.; Badoei-Dalfard, A.; Khajeh, K.
    Enhanced expression of a recombinant bacterial laccase at low temperature and microaerobic conditions: purification and biochemical characterization (2010), J. Ind. Microbiol. Biotechnol., 37, 863-869.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
syringaldazine at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)
0.053
-
2,6-dimethoxyphenol at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)
0.535
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) HR03
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus sp. (in: Bacteria) HR03 ?
-
?
2,6-dimethoxyphenol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
2,6-dimethoxyphenol + O2
-
Bacillus sp. (in: Bacteria) HR03 ?
-
?
syringaldazine + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
syringaldazine + O2
-
Bacillus sp. (in: Bacteria) HR03 ?
-
?

Synonyms

Synonyms Comment Organism
benzenediol oxygen oxidoreductase
-
Bacillus sp. (in: Bacteria)
CotA
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 with syringaldazine as the substrate, the activity is increased 3fold after 50 min preincubation at 70°C and 2.4fold after 10 min preincubation at 80°C. Preincubation of the enzyme in 70°C for 30 min raises the activity 4fold with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as the substrate Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
2,6-dimethoxyphenol at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)
20
-
syringaldazine at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)
127
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at 22°C, pH not specified in the publication Bacillus sp. (in: Bacteria)