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Literature summary for 1.10.3.2 extracted from

  • Niladevi, K.; Jacob, N.; Prema, P.
    Evidence for a halotolerant-alkaline laccase in Streptomyces psammoticus: Purification and characterization (2008), Process Biochem., 43, 654-660.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 21% inhibition at 1 mM Kitasatospora aureofaciens
Co2+ 29% inhibition at 1 mM Kitasatospora aureofaciens
DTT complete inhibition at 0.1 mM Kitasatospora aureofaciens
EDTA 87% inhibition at 1 mM, complete inhibition at 2 mM Kitasatospora aureofaciens
Hg2+ 14% inhibition at 1 mM Kitasatospora aureofaciens
kojic acid 47% inhibition at 1 mM Kitasatospora aureofaciens
L-cysteine 89% inhibition at 0.1 mM, complete inhibition at 1 mM Kitasatospora aureofaciens
Mn2+ 22% inhibition at 1 mM Kitasatospora aureofaciens
NaN3 complete inhibition at 0.1 mM Kitasatospora aureofaciens
Ni2+ 32% inhibition at 1 mM Kitasatospora aureofaciens
thioglycolic acid 93% inhibition at 0.1 mM, complete inhibition at 1 mM Kitasatospora aureofaciens
Thiourea 73% inhibition at 5 mM Kitasatospora aureofaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates, 148% at 2 mM Kitasatospora aureofaciens
Fe2+ activates, 204% at 2 mM Kitasatospora aureofaciens
Mg2+ activates, 137% at 2 mM Kitasatospora aureofaciens
additional information the enzyme activity is not affected by Ca2+ at 2 mM, and by NaCl up to 1.2 M Kitasatospora aureofaciens
Zn2+ activates, 146% at 2 mM Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Kitasatospora aureofaciens
70000 75000 gel filtration Kitasatospora aureofaciens

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
-
-
Kitasatospora aureofaciens MTCC 7334
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 12.1fold to homogeneity by anion exchange chromatography and gel filtration Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.6
-
purified enzyme Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,8-diaminonaphthalene + O2
-
Kitasatospora aureofaciens ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Kitasatospora aureofaciens ?
-
?
2,6-dimethoxyphenol + O2
-
Kitasatospora aureofaciens ?
-
?
catechol + O2
-
Kitasatospora aureofaciens ?
-
?
catechol + O2
-
Kitasatospora aureofaciens MTCC 7334 ?
-
?
dopa + O2
-
Kitasatospora aureofaciens ?
-
?
ferulic acid + O2
-
Kitasatospora aureofaciens ?
-
?
gallic acid + O2
-
Kitasatospora aureofaciens ?
-
?
gallic acid + O2
-
Kitasatospora aureofaciens MTCC 7334 ?
-
?
guaiacol + O2
-
Kitasatospora aureofaciens ?
-
?
additional information substrate specificity, no activity with L-tyrosine, overview Kitasatospora aureofaciens ?
-
?
additional information substrate specificity, no activity with L-tyrosine, overview Kitasatospora aureofaciens MTCC 7334 ?
-
?
pyrogallol + O2
-
Kitasatospora aureofaciens ?
-
?
pyrogallol + O2
-
Kitasatospora aureofaciens MTCC 7334 ?
-
?
resorcinol + O2
-
Kitasatospora aureofaciens ?
-
?
syringaldazine + O2
-
Kitasatospora aureofaciens ?
-
?
syringaldazine + O2
-
Kitasatospora aureofaciens MTCC 7334 ?
-
?
tannic acid + O2
-
Kitasatospora aureofaciens ?
-
?
vanillic acid + O2
-
Kitasatospora aureofaciens ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Kitasatospora aureofaciens

Synonyms

Synonyms Comment Organism
More the enzyme is a typical blue laccase Kitasatospora aureofaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Kitasatospora aureofaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 40% of maximal activity at 25°C, maximal activity at 45°C, 90% of maximal activity at 50°C, 85% of maximal activity at 55°C, and 69% at 60°C Kitasatospora aureofaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 purified enzyme, 90 min, completely stable Kitasatospora aureofaciens
60
-
purified enzyme, 90 min, 65% remaining activity Kitasatospora aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Kitasatospora aureofaciens

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, 60-65% of maximal activity at pH 4.0 and pH 10.0, profile, overview Kitasatospora aureofaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 purified enzyme, 90 min, stable at pH 6.5-9.5, 20% remaining activity at pH 2.0, 65% remaining activity at pH 4.0 and pH 10.0 Kitasatospora aureofaciens

pI Value

Organism Comment pI Value Maximum pI Value
Kitasatospora aureofaciens isoelectric focussing
-
7.9