Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 21% inhibition at 1 mM | Kitasatospora aureofaciens | |
Co2+ | 29% inhibition at 1 mM | Kitasatospora aureofaciens | |
DTT | complete inhibition at 0.1 mM | Kitasatospora aureofaciens | |
EDTA | 87% inhibition at 1 mM, complete inhibition at 2 mM | Kitasatospora aureofaciens | |
Hg2+ | 14% inhibition at 1 mM | Kitasatospora aureofaciens | |
kojic acid | 47% inhibition at 1 mM | Kitasatospora aureofaciens | |
L-cysteine | 89% inhibition at 0.1 mM, complete inhibition at 1 mM | Kitasatospora aureofaciens | |
Mn2+ | 22% inhibition at 1 mM | Kitasatospora aureofaciens | |
NaN3 | complete inhibition at 0.1 mM | Kitasatospora aureofaciens | |
Ni2+ | 32% inhibition at 1 mM | Kitasatospora aureofaciens | |
thioglycolic acid | 93% inhibition at 0.1 mM, complete inhibition at 1 mM | Kitasatospora aureofaciens | |
Thiourea | 73% inhibition at 5 mM | Kitasatospora aureofaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates, 148% at 2 mM | Kitasatospora aureofaciens | |
Fe2+ | activates, 204% at 2 mM | Kitasatospora aureofaciens | |
Mg2+ | activates, 137% at 2 mM | Kitasatospora aureofaciens | |
additional information | the enzyme activity is not affected by Ca2+ at 2 mM, and by NaCl up to 1.2 M | Kitasatospora aureofaciens | |
Zn2+ | activates, 146% at 2 mM | Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Kitasatospora aureofaciens |
70000 | 75000 | gel filtration | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Kitasatospora aureofaciens MTCC 7334 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 12.1fold to homogeneity by anion exchange chromatography and gel filtration | Kitasatospora aureofaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.6 | - |
purified enzyme | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,8-diaminonaphthalene + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
catechol + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
catechol + O2 | - |
Kitasatospora aureofaciens MTCC 7334 | ? | - |
? | |
dopa + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
ferulic acid + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
gallic acid + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
gallic acid + O2 | - |
Kitasatospora aureofaciens MTCC 7334 | ? | - |
? | |
guaiacol + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
additional information | substrate specificity, no activity with L-tyrosine, overview | Kitasatospora aureofaciens | ? | - |
? | |
additional information | substrate specificity, no activity with L-tyrosine, overview | Kitasatospora aureofaciens MTCC 7334 | ? | - |
? | |
pyrogallol + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
pyrogallol + O2 | - |
Kitasatospora aureofaciens MTCC 7334 | ? | - |
? | |
resorcinol + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
syringaldazine + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
syringaldazine + O2 | - |
Kitasatospora aureofaciens MTCC 7334 | ? | - |
? | |
tannic acid + O2 | - |
Kitasatospora aureofaciens | ? | - |
? | |
vanillic acid + O2 | - |
Kitasatospora aureofaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Kitasatospora aureofaciens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a typical blue laccase | Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Kitasatospora aureofaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | 40% of maximal activity at 25°C, maximal activity at 45°C, 90% of maximal activity at 50°C, 85% of maximal activity at 55°C, and 69% at 60°C | Kitasatospora aureofaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified enzyme, 90 min, completely stable | Kitasatospora aureofaciens |
60 | - |
purified enzyme, 90 min, 65% remaining activity | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Kitasatospora aureofaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, 60-65% of maximal activity at pH 4.0 and pH 10.0, profile, overview | Kitasatospora aureofaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | purified enzyme, 90 min, stable at pH 6.5-9.5, 20% remaining activity at pH 2.0, 65% remaining activity at pH 4.0 and pH 10.0 | Kitasatospora aureofaciens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kitasatospora aureofaciens | isoelectric focussing | - |
7.9 |