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Literature summary for 1.10.3.2 extracted from
- Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye (2008), Appl. Microbiol. Biotechnol., 79, 597-603.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the enzyme may be used in a variety of biotechnological applications, including textile dye bleaching, pulp bleaching, bioremediation, polymer synthesis and biosensors | Streptomyces coelicolor |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Streptomyces lividans, the enzyme is secreted | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by EDTA at 1-10 mM | Streptomyces coelicolor | |
| NaN3 | 22% inhibition at 10 mM, no inhibition by 1 mM NaN3 | Streptomyces coelicolor |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a copper-containing enzyme | Streptomyces coelicolor | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracellular recombinant enzyme expressed in Streptomyces lividans to homogeneity | Streptomyces coelicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
purified recombinant enzyme, with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid at pH 4.0 | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
| syringaldazine + O2 | the enzyme is able to rapidly decolourize the dye Indigo carmine in the presence of syringaldehyde as a redox mediator, at pH 9.0 and 45°C | Streptomyces coelicolor | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SLAC | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at pH 4.0 | Streptomyces coelicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Streptomyces coelicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified recombinant enzyme, 90% remaining activity after 25 min, 50% remaining actiivty after 110 min | Streptomyces coelicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Streptomyces coelicolor |
| 9 | - |
assay at, substrate 2,6-dimethoxyphenol | Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | activity range, dependent on the substrate | Streptomyces coelicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | purified recombinant enzyme, 48 h, 30°C, completely stable | Streptomyces coelicolor |
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