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Literature summary for 1.10.3.2 extracted from

  • Papinutti, L.; Martinez, M.J.
    Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus (2006), J. Chem. Technol. Biotechnol., 81, 1064-1070.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ stimulates, induction Fomes sclerodermeus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information both isozymes show Michaelis-Menten kinetics, overview Fomes sclerodermeus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
x * 67000, both isozymes, SDS-PAGE Fomes sclerodermeus

Organism

Organism UniProt Comment Textmining
Fomes sclerodermeus
-
two isozymes Lac I and Lac II
-
Fomes sclerodermeus BAFC 2752
-
two isozymes Lac I and Lac II
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3% N-linked carbohydrate content for both isozymes Fomes sclerodermeus

Purification (Commentary)

Purification (Comment) Organism
native isozymes Lac I and II by anion exchange chromatography and gel filtration, overview Fomes sclerodermeus

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme production in presence or absence of exogenous copper, overview Fomes sclerodermeus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Fomes sclerodermeus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Fomes sclerodermeus ?
-
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Fomes sclerodermeus BAFC 2752 ?
-
?
2,6-dimethoxyphenol + O2 preferred substrate Fomes sclerodermeus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
2,6-dimethoxyphenol + O2 preferred substrate Fomes sclerodermeus BAFC 2752 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
ferulic acid + O2
-
Fomes sclerodermeus ?
-
?
ferulic acid + O2
-
Fomes sclerodermeus BAFC 2752 ?
-
?
vanillic acid + O2
-
Fomes sclerodermeus ?
-
?
vanillic acid + O2
-
Fomes sclerodermeus BAFC 2752 ?
-
?
veratric acid + O2
-
Fomes sclerodermeus ?
-
?
veratric acid + O2
-
Fomes sclerodermeus BAFC 2752 ?
-
?

Subunits

Subunits Comment Organism
? x * 67000, both isozymes, SDS-PAGE Fomes sclerodermeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
182
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, isozyme Lac II Fomes sclerodermeus
199
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, isozyme Lac I Fomes sclerodermeus
206
-
2,6-dimethoxyphenol pH 5.0, isozyme Lac I Fomes sclerodermeus
218
-
2,6-dimethoxyphenol pH 5.0, isozyme Lac II Fomes sclerodermeus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Fomes sclerodermeus

pI Value

Organism Comment pI Value Maximum pI Value
Fomes sclerodermeus Lac I, isoelectric focusing
-
3.41
Fomes sclerodermeus Lac II, isoelectric focusing
-
3.48