Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Pleurotus eryngii |
68000 | - |
gel filtration | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pleurotus eryngii | the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme about 35fold from fruiting bodies by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration | Pleurotus eryngii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Pleurotus eryngii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM | Pleurotus eryngii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Pleurotus eryngii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pleurotus eryngii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | high activity at 80°C, optimum at 70°C, constant rise of activity from 20°C to 70°C | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pleurotus eryngii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | abrupt activity decline at pH 6.0, indiscernible at pH 8.0-9.0 | Pleurotus eryngii |