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Literature summary for 1.10.3.2 extracted from
- Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea (2004), Eur. J. Biochem., 271, 318-328.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Volvariella volvacea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | 0.1 mM, 100% inhibition | Volvariella volvacea |  |
| cysteine | 0.1 mM, 100% inhibition | Volvariella volvacea | |
| dithiothreitol | 0.1 mM, 100% inhibition | Volvariella volvacea | |
| EDTA | 1 mM, 20% inhibition | Volvariella volvacea | |
| thioglycolic acid | 1 mM, 100% inhibition | Volvariella volvacea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
syringaldazine | pH 5.6, 45°C | Volvariella volvacea | |
| 0.03 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 45°C | Volvariella volvacea | |
| 0.57 | - |
2,6-dimethoxyphenol | pH 4.6, 45°C | Volvariella volvacea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, SDS-PAGE | Volvariella volvacea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Volvariella volvacea | - |
growth medium supplemented with copper | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Volvariella volvacea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Volvariella volvacea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
substrate 2,6-dimethoxyphenol, pH 4.6 | Volvariella volvacea |
| 3.4 | - |
substrate syringaldazine, pH 5.6 | Volvariella volvacea |
| 13.5 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0 | Volvariella volvacea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Volvariella volvacea | ? + H2O | - |
? | |
| 2,6-dimethoxyphenol + H2O | about 5% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea | ? | - |
? | |
| additional information | poor substrates: guaiacol, catechol, no substrate: tyrosine, L-3,4-dimethoxyphenol, dihydroxyphenylalanine | Volvariella volvacea | ? | - |
? | |
| syringaldazine + O2 | about 17% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Volvariella volvacea |
| More | N-terminal amino acid sequence | Volvariella volvacea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea |
| 4.6 | - |
substrate 2,6-dimethoxyphenol | Volvariella volvacea |
| 5.6 | - |
substrate syringaldazine | Volvariella volvacea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Volvariella volvacea | isoelectric focusing | - |
4.2 |
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