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Literature summary for 1.10.3.2 extracted from

  • Farnet, A.M.; Criquet, S.; Cigna, M.; Gil, G.; Ferre, E.
    Purification of a laccase from Marasmius quercophilus induced with ferulic acid: reactivity towards natural and xenobiotic aromatic compounds (2004), Enzyme Microb. Technol., 34, 549-554.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
azide strong Gymnopus quercophilus
additional information not inhibitory: EDTA. L-cysteine Gymnopus quercophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
syringaldazine pH 4.5 Gymnopus quercophilus
0.0113
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Gymnopus quercophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Gymnopus quercophilus

Organism

Organism UniProt Comment Textmining
Gymnopus quercophilus
-
induction with ferulic acid, three isozymes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Gymnopus quercophilus ? + H2O
-
?
2,4,6-trichlorophenol + O2
-
Gymnopus quercophilus ?
-
?
2,4-dichlorophenol + O2
-
Gymnopus quercophilus ?
-
?
2-chlorophenol + O2
-
Gymnopus quercophilus ?
-
?
syringaldazine + O2
-
Gymnopus quercophilus ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Gymnopus quercophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
24h, 100% residual activity, increase in activity by 80% after shift from room temperature to 30°C Gymnopus quercophilus
40
-
24h, 100% residual activity, increase in activity by 100% after shift from room temperature to 40°C Gymnopus quercophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Gymnopus quercophilus
4.5
-
substrate syringaldazine Gymnopus quercophilus