Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Min, K.L.; Kim, Y.H.; Kim, Y.W.; Jung, H.S.; Hah, Y.C.
    Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis (2001), Arch. Biochem. Biophys., 392, 279-286.
    View publication on PubMed
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
H2O2 slight activation, increased by Mn2+ Phellinus ribis
Mn2+ activation in presence of H2O2 Phellinus ribis

Inhibitors

Inhibitors Comment Organism Structure
azide 85% inhibition at 1 mM Phellinus ribis
thioglycolic acid 85% inhibition at 1 mM Phellinus ribis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 syringaldazine
-
 Phellinus ribis
0.038
-
 2,6-dimethoxyphenol
-
 Phellinus ribis
0.21
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
 Phellinus ribis

Metals/Ions

Metals/Ions Comment Organism Structure
copper one gatom per mol Phellinus ribis
Manganese 1 gatom per mol Phellinus ribis
Zinc 2 gatom per mol Phellinus ribis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 gel filtration Phellinus ribis
76000
-
 2 * 76000, SDS-PAGE, N-terminal amino acid sequence Phellinus ribis

Organism

Organism UniProt Comment Textmining
Phellinus ribis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sugar content 28% Phellinus ribis

Purification (Commentary)

Purification (Comment) Organism
-
 Phellinus ribis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
 Phellinus ribis 2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Phellinus ribis ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Phellinus ribis ? + H2O
-
 ?
ferulic acid + O2
-
 Phellinus ribis 3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
guaiacol + O2
-
 Phellinus ribis 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
o-dianisidine
-
 Phellinus ribis ?
-
 ?
sinapic acid + O2
-
 Phellinus ribis 3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
 ?
sinapic acid + O2
-
 Phellinus ribis ? + H2O
-
 ?
syringaldazine + O2
-
 Phellinus ribis ?
-
 ?
vanillic acid + O2
-
 Phellinus ribis 4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 76000, SDS-PAGE, N-terminal amino acid sequence Phellinus ribis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6
-
 Phellinus ribis