Application | Comment | Organism |
---|---|---|
food industry | inhibition of enzyme PPO is important in the food industry, due its role in browning | Physalis peruviana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Physalis peruviana | |
CaCl2 | - |
Physalis peruviana | |
Citric acid | weak inhibition | Physalis peruviana | |
EDTA | - |
Physalis peruviana | |
fukugiside | - |
Physalis peruviana | |
hydroquinone | - |
Physalis peruviana | |
L-cysteine | - |
Physalis peruviana | |
additional information | no inhibition by amentoflavone, BHT, and morelloflavone | Physalis peruviana | |
NaCl | - |
Physalis peruviana | |
quercetin | - |
Physalis peruviana | |
sodium sulfite | - |
Physalis peruviana | |
Tannic acid | - |
Physalis peruviana | |
tartaric acid | - |
Physalis peruviana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
chlorogenic acid | pH 5.0, 20°C | Physalis peruviana | |
3.88 | - |
4-methylcatechol | pH 5.0, 20°C | Physalis peruviana | |
203.8 | - |
catechol | pH 5.0, 20°C | Physalis peruviana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-methylcatechol + O2 | Physalis peruviana | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 4-methylcatechol + O2 | Physalis peruviana Colombian ecotype | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | Physalis peruviana | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | Physalis peruviana Colombian ecotype | - |
2 1,2-benzoquinone + 2 H2O | - |
? | |
chlorogenic acid + O2 | Physalis peruviana | best substrate | ? | - |
? | |
chlorogenic acid + O2 | Physalis peruviana Colombian ecotype | best substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physalis peruviana | - |
- |
- |
Physalis peruviana Colombian ecotype | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 170fold from fruits by acetone and ATPS precipitation | Physalis peruviana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | the activity (measured with 4-methylcatechol as substrate) decreases during fruit ripening, overview | Physalis peruviana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
204.69 | - |
purified enzyme, substrate chlorogenic acid, pH 5.0, 20°C | Physalis peruviana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-methylcatechol + O2 | - |
Physalis peruviana | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 4-methylcatechol + O2 | - |
Physalis peruviana Colombian ecotype | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | - |
Physalis peruviana | 2 1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | - |
Physalis peruviana Colombian ecotype | 2 1,2-benzoquinone + 2 H2O | - |
? | |
chlorogenic acid + O2 | best substrate | Physalis peruviana | ? | - |
? | |
chlorogenic acid + O2 | best substrate | Physalis peruviana Colombian ecotype | ? | - |
? | |
additional information | the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 | Physalis peruviana | ? | - |
? | |
additional information | the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 | Physalis peruviana Colombian ecotype | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Physalis peruviana |
PPO | - |
Physalis peruviana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
with chlorogenic acid | Physalis peruviana |
25 | - |
with 4-methylcatechol | Physalis peruviana |
40 | - |
with catechol | Physalis peruviana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | pH profiles withdifferent substrates, overview | Physalis peruviana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20.8 | - |
kinetic parameters for the thermal inactivation of Cape gooseberry PPO, overview | Physalis peruviana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
with chlorogenic acid | Physalis peruviana |
5.5 | - |
with catechol and 4-methylcatechol | Physalis peruviana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.6 | 6.5 | pH profiles with different substrates, overview | Physalis peruviana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | quercetin | |
0.044 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | L-cysteine | |
0.044 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | hydroquinone | |
0.045 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | ascorbate | |
0.049 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | quercetin | |
0.052 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | L-cysteine | |
0.06 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | ascorbate | |
0.062 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | Tannic acid | |
0.15 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | sodium sulfite | |
0.157 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | sodium sulfite | |
0.372 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | Tannic acid | |
0.536 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | fukugiside | |
0.563 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | hydroquinone | |
0.632 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | fukugiside | |
3.56 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | CaCl2 | |
5.01 | - |
pH 5.5, 25°C, with chlorogenic acid | Physalis peruviana | NaCl | |
30.96 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | CaCl2 | |
39.54 | - |
pH 5.5, 25°C, with 4-methylcatechol | Physalis peruviana | NaCl |