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Literature summary for 1.10.3.1 extracted from

  • Bravo, K.; Osorio, E.
    Characterization of polyphenol oxidase from Cape gooseberry (Physalis peruviana L.) fruit (2016), Food Chem., 197, 185-190 .
    View publication on PubMed

Application

Application Comment Organism
food industry inhibition of enzyme PPO is important in the food industry, due its role in browning Physalis peruviana

Inhibitors

Inhibitors Comment Organism Structure
ascorbate
-
Physalis peruviana
CaCl2
-
Physalis peruviana
Citric acid weak inhibition Physalis peruviana
EDTA
-
Physalis peruviana
fukugiside
-
Physalis peruviana
hydroquinone
-
Physalis peruviana
L-cysteine
-
Physalis peruviana
additional information no inhibition by amentoflavone, BHT, and morelloflavone Physalis peruviana
NaCl
-
Physalis peruviana
quercetin
-
Physalis peruviana
sodium sulfite
-
Physalis peruviana
Tannic acid
-
Physalis peruviana
tartaric acid
-
Physalis peruviana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
chlorogenic acid pH 5.0, 20°C Physalis peruviana
3.88
-
4-methylcatechol pH 5.0, 20°C Physalis peruviana
203.8
-
catechol pH 5.0, 20°C Physalis peruviana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-methylcatechol + O2 Physalis peruviana
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 4-methylcatechol + O2 Physalis peruviana Colombian ecotype
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2 Physalis peruviana
-
2 1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2 Physalis peruviana Colombian ecotype
-
2 1,2-benzoquinone + 2 H2O
-
?
chlorogenic acid + O2 Physalis peruviana best substrate ?
-
?
chlorogenic acid + O2 Physalis peruviana Colombian ecotype best substrate ?
-
?

Organism

Organism UniProt Comment Textmining
Physalis peruviana
-
-
-
Physalis peruviana Colombian ecotype
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 170fold from fruits by acetone and ATPS precipitation Physalis peruviana

Source Tissue

Source Tissue Comment Organism Textmining
fruit the activity (measured with 4-methylcatechol as substrate) decreases during fruit ripening, overview Physalis peruviana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
204.69
-
purified enzyme, substrate chlorogenic acid, pH 5.0, 20°C Physalis peruviana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-methylcatechol + O2
-
Physalis peruviana 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 4-methylcatechol + O2
-
Physalis peruviana Colombian ecotype 2 4-methyl-1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2
-
Physalis peruviana 2 1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2
-
Physalis peruviana Colombian ecotype 2 1,2-benzoquinone + 2 H2O
-
?
chlorogenic acid + O2 best substrate Physalis peruviana ?
-
?
chlorogenic acid + O2 best substrate Physalis peruviana Colombian ecotype ?
-
?
additional information the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 Physalis peruviana ?
-
?
additional information the specificity of PPO toward chlorogenic acid is approximately 7 times greater than that for 4-methylcatechol and 370 times greater than that for catechol. No activity with L-tyrosine. The Cape gooseberry PPO lacks monophenolase (cresolase) activity, EC 1.14.18.1 Physalis peruviana Colombian ecotype ?
-
?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Physalis peruviana
PPO
-
Physalis peruviana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
with chlorogenic acid Physalis peruviana
25
-
with 4-methylcatechol Physalis peruviana
40
-
with catechol Physalis peruviana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 pH profiles withdifferent substrates, overview Physalis peruviana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20.8
-
kinetic parameters for the thermal inactivation of Cape gooseberry PPO, overview Physalis peruviana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
with chlorogenic acid Physalis peruviana
5.5
-
with catechol and 4-methylcatechol Physalis peruviana

pH Range

pH Minimum pH Maximum Comment Organism
3.6 6.5 pH profiles with different substrates, overview Physalis peruviana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana quercetin
0.044
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana L-cysteine
0.044
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana hydroquinone
0.045
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana ascorbate
0.049
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana quercetin
0.052
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana L-cysteine
0.06
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana ascorbate
0.062
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana Tannic acid
0.15
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana sodium sulfite
0.157
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana sodium sulfite
0.372
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana Tannic acid
0.536
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana fukugiside
0.563
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana hydroquinone
0.632
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana fukugiside
3.56
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana CaCl2
5.01
-
pH 5.5, 25°C, with chlorogenic acid Physalis peruviana NaCl
30.96
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana CaCl2
39.54
-
pH 5.5, 25°C, with 4-methylcatechol Physalis peruviana NaCl