Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.1 extracted from

  • Gul Guven, R.; Aslan, N.; Guven, K.; Matpan Bekler, F.; Acer, O.
    Purification and characterization of polyphenol oxidase from corn tassel (2016), Cell. Mol. Biol., 62, 6-11 .
    View publication on PubMed
Search for more literature for 1.10.3.1

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 17% activation at 2 mM Zea mays
D-glucose 16% activation at 2 mM Zea mays
sucrose 4% activation at 2 mM Zea mays

Application

Application Comment Organism
food industry inhibition of catechol oxidase diminishes browning in white radish and elevates food quality Zea mays

Inhibitors

Inhibitors Comment Organism Structure
EDTA 22.2% inhibition at 2 mM Zea mays
SDS 12.2% inhibition at 2 mM Zea mays
Sodium azide 26.6% inhibition at 2 mM Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Zea mays
3.48
-
 catechol pH 8.0, 40°C Zea mays
8
-
 4-methylcatechol pH 6.0, 30°C Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
158000
-
 native PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from corn tassel 10fold by ammonium sulfate fractionation and gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
tassel
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 2612.06 U/mg, one enzyme unit is defined as the amount of enzyme that produces a rise of 0.001 absorbance in one minute at 420 nm Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-methylcatechol + O2
-
 Zea mays 2 4-methyl-1,2-benzoquinone
-
 ?
2 catechol + O2 best substrate Zea mays 2 1,2-benzoquinone + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 158000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Zea mays
PPO
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 with substrate 4-methylcatechol Zea mays
40
-
 with substrate catechol Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 with substrate 4-methylcatechol Zea mays
8
-
 with substrate catechol Zea mays