Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cv. BramleyÂ’s seedling | - |
Purification (Comment) | Organism |
---|---|
native enzyme 75.5fold to homogeneity from apples purification in presence of AEBSF and aprotinin by ammonium sulfate fractionation, dialysis, gel filtration, and hydrophobic interaction chromatography, followed by anion exchange and hydroxyapatite chromatography | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | ripe | Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
227700 | - |
highly purified enzyme, substrate catechol | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-epicatechin + O2 | - |
Malus domestica | ? | - |
? | |
4-methylcatechol + O2 | - |
Malus domestica | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
caffeic acid + O2 | - |
Malus domestica | ? | - |
? | |
catechol + 1/2 O2 | - |
Malus domestica | 1,2-benzoquinone + H2O | - |
? | |
DL-DOPA + O2 | - |
Malus domestica | dopaquinone + H2O | - |
? | |
additional information | substrate specificity of the purified enzyme, overview, highest PPO activity occurs with 4-methylcatechol, followed by catechol, pyrogallol, (-)-epicatechin, caffeic acid, and DL-dopa, little or no activity is detected toward the monophenolic compounds ferulic acid, L-tyrosine, and phenol | Malus domestica | ? | - |
? | |
pyrogallol + O2 | - |
Malus domestica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Malus domestica |
PPO | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
with substrate catechol | Malus domestica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Malus domestica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | 72 h, purified enzyme, over 50% activity remaining | Malus domestica |
40 | 50 | 24 h, purified enzyme, over 50% activity remaining | Malus domestica |
60 | - |
1 h, purified enzyme, over 50% activity remaining | Malus domestica |
70 | - |
15 min, purified enzyme, over 50% activity remaining | Malus domestica |
80 | - |
15 min, purified enzyme, inactivation | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
with substrate catechol | Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 9 | - |
Malus domestica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 3.5 | purified enzyme, most unstable at, less than 10% activity remaining after 2 h | Malus domestica |
4.5 | - |
purified enzyme, over 50% activity reamining after 24 h | Malus domestica |
5 | 7.5 | purified enzyme, over 50% activity reamining after 48 h | Malus domestica |
5.6 | 6.5 | purified enzyme, most stable at, over 50% remaining activity after 72 h | Malus domestica |
8 | 8.5 | purified enzyme, over 50% activity reamining after 24 h | Malus domestica |
9 | - |
purified enzyme, over 50% activity reamining after 4 h | Malus domestica |