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Literature summary for 1.10.3.1 extracted from

  • Ni Eidhinn, D.M.; Murphy, E.; O'Beirne, D.
    Polyphenol oxidase from apple (Malus domestica Borkh. cv Bramley’s seedling): purification strategies and characterization (2006), J. Food Sci., 71, C51-C58.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Malus domestica
-
cv. BramleyÂ’s seedling
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 75.5fold to homogeneity from apples purification in presence of AEBSF and aprotinin by ammonium sulfate fractionation, dialysis, gel filtration, and hydrophobic interaction chromatography, followed by anion exchange and hydroxyapatite chromatography Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
fruit ripe Malus domestica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
227700
-
highly purified enzyme, substrate catechol Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-epicatechin + O2
-
Malus domestica ?
-
?
4-methylcatechol + O2
-
Malus domestica 4-methyl-1,2-benzoquinone + H2O
-
?
caffeic acid + O2
-
Malus domestica ?
-
?
catechol + 1/2 O2
-
Malus domestica 1,2-benzoquinone + H2O
-
?
DL-DOPA + O2
-
Malus domestica dopaquinone + H2O
-
?
additional information substrate specificity of the purified enzyme, overview, highest PPO activity occurs with 4-methylcatechol, followed by catechol, pyrogallol, (-)-epicatechin, caffeic acid, and DL-dopa, little or no activity is detected toward the monophenolic compounds ferulic acid, L-tyrosine, and phenol Malus domestica ?
-
?
pyrogallol + O2
-
Malus domestica ?
-
?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Malus domestica
PPO
-
Malus domestica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
with substrate catechol Malus domestica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Malus domestica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 72 h, purified enzyme, over 50% activity remaining Malus domestica
40 50 24 h, purified enzyme, over 50% activity remaining Malus domestica
60
-
1 h, purified enzyme, over 50% activity remaining Malus domestica
70
-
15 min, purified enzyme, over 50% activity remaining Malus domestica
80
-
15 min, purified enzyme, inactivation Malus domestica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
with substrate catechol Malus domestica

pH Range

pH Minimum pH Maximum Comment Organism
2.5 9
-
Malus domestica

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 3.5 purified enzyme, most unstable at, less than 10% activity remaining after 2 h Malus domestica
4.5
-
purified enzyme, over 50% activity reamining after 24 h Malus domestica
5 7.5 purified enzyme, over 50% activity reamining after 48 h Malus domestica
5.6 6.5 purified enzyme, most stable at, over 50% remaining activity after 72 h Malus domestica
8 8.5 purified enzyme, over 50% activity reamining after 24 h Malus domestica
9
-
purified enzyme, over 50% activity reamining after 4 h Malus domestica