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Literature summary for 1.1.99.B9 extracted from

  • Xue, Y.P.; Tian, F.F.; Ruan, L.T.; Liu, Z.Q.; Zheng, Y.G.; Shen, Y.C.
    Concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by asymmetric oxidation with a newly isolated Pseudomonas aeruginosa ZJB1125 (2013), J. Biotechnol., 167, 271-278.
    View publication on PubMed
Search for more literature for 1.1.99.B9

Application

Application Comment Organism
synthesis enzyme can be used for the concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by oxidation of aromatic 2-hydroxyacids in one-step biotransformation Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa ZJB1125
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-(2-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (2-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(2-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa ZJB1125 (2-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(2-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (2-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(2-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa ZJB1125 (2-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(3-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (3-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(3-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa ZJB1125 (3-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(4-bromophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (4-bromophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(4-bromophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa ZJB1125 (4-bromophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(4-chlorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (4-chlorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-(4-fluorophenyl)(hydroxy)acetic acid + acceptor
-
 Pseudomonas aeruginosa (4-fluorophenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-hydroxy(4-hydroxyphenyl)acetic acid + acceptor
-
 Pseudomonas aeruginosa (4-hydroxyphenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-hydroxy(4-methylphenyl)acetic acid + acceptor
-
 Pseudomonas aeruginosa (4-methylphenyl)(oxo)acetic acid + reduced acceptor
-
 ?
(2S)-hydroxy(phenyl)acetic acid + acceptor
-
 Pseudomonas aeruginosa oxo(phenyl)acetic acid + reduced acceptor
-
 ?
additional information enzyme is a stereoselective 2-hydroxyacid dehydrogenase (2-HADH) that can catalyze asymmetric oxidation of mandelic acid and 2-chloromandelic acid into corresponding 2-ketoacids and (R)-isomers with high activity and enantioselectivity, while no consecutive oxidation of 2-ketoacids is observed Pseudomonas aeruginosa ?
-
 ?
additional information enzyme is a stereoselective 2-hydroxyacid dehydrogenase (2-HADH) that can catalyze asymmetric oxidation of mandelic acid and 2-chloromandelic acid into corresponding 2-ketoacids and (R)-isomers with high activity and enantioselectivity, while no consecutive oxidation of 2-ketoacids is observed Pseudomonas aeruginosa ZJB1125 ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Pseudomonas aeruginosa
additional information does not require NAD(P)+ as cofactors to catalyze the oxidation reaction Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa presence of 2-chloromandelic acid induces enzyme synthesis efficiently additional information