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Literature summary for 1.1.99.B3 extracted from

  • Foumani, M.; Vuong, T.V.; Master, E.R.
    Altered substrate specificity of the gluco-oligosaccharide oxidase from Acremonium strictum (2011), Biotechnol. Bioeng., 108, 2261-2269.
    View publication on PubMed
Search for more literature for 1.1.99.B3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Sarocladium strictum

Protein Variants

Protein Variants Comment Organism
W351F the mutant shows reduced kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum
Y300A the mutation doubles kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum
Y300N the mutation doubles kcat values for monosaccharide and oligosaccharide substrates Sarocladium strictum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.083
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.085
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.098
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.1
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.11
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.25
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
0.25
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
0.31
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.35
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.38
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
0.44
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
2.81
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
3.11
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
3.15
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
4.3
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
4.83
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
5
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
5.11
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
8.1
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
11
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
17.4
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
19.6
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
31
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
32
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
36.1
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
51.8
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
56
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
92
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
96
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
105
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
110
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
132
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
288
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
340
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
950
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
cellobiose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
cellotriose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
cellotriose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
D-galactose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
D-glucose + O2 Sarocladium strictum highest catalytic efficiency with D-glucose ? + H2O2
-
 ?
D-xylose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
D-xylose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
maltose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
maltose + O2 Sarocladium strictum CBS 346.70
-
 ? + H2O2
-
 ?
additional information Sarocladium strictum no activity with mannose ?
-
 ?
additional information Sarocladium strictum CBS 346.70 no activity with mannose ?
-
 ?
N-acetyl-D-glucosamine + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
xylobiose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?
xylotriose + O2 Sarocladium strictum
-
 ? + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Sarocladium strictum Q6PW77
-
-
Sarocladium strictum CBS 346.70 Q6PW77
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Sarocladium strictum

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:wheat bran-grown cell
-
 Sarocladium strictum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
cellobiose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
cellotriose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
cellotriose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
D-galactose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
D-glucose + O2 highest catalytic efficiency with D-glucose Sarocladium strictum ? + H2O2
-
 ?
D-xylose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
D-xylose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
maltose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
maltose + O2
-
 Sarocladium strictum CBS 346.70 ? + H2O2
-
 ?
additional information no activity with mannose Sarocladium strictum ?
-
 ?
additional information no activity with mannose Sarocladium strictum CBS 346.70 ?
-
 ?
N-acetyl-D-glucosamine + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
xylobiose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?
xylotriose + O2
-
 Sarocladium strictum ? + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
gluco-oligosaccharide oxidase
-
 Sarocladium strictum
GOOX
-
 Sarocladium strictum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
5.3
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
5.3
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
5.4
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
5.6
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
5.7
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
6
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
6
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
6.3
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
6.6
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
7.2
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
7.5
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
7.8
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
7.9
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
8
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
8.1
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
8.3
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
8.8
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
10
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
10.4
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
10.4
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
10.8
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
11.2
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
11.3
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
11.3
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
11.4
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
11.7
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
11.8
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
12
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
12
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
12.8
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
13.2
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
13.3
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
13.3
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
13.7
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
13.9
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 N-acetyl-D-glucosamine mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.02
-
 D-xylose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.02
-
 N-acetyl-D-glucosamine wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.05
-
 D-xylose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.06
-
 D-galactose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.11
-
 D-galactose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
0.14
-
 D-galactose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
0.14
-
 N-acetyl-D-glucosamine mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
0.18
-
 D-glucose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.18
-
 D-galactose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
0.2
-
 N-acetyl-D-glucosamine mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
0.22
-
 D-xylose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
0.36
-
 D-xylose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
0.43
-
 D-glucose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
0.53
-
 maltose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
0.95
-
 maltose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
1.1
-
 maltose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
1.63
-
 D-glucose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
2.13
-
 maltose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
2.48
-
 xylobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
2.6
-
 xylobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
2.83
-
 xylotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
3.6
-
 D-glucose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
4.33
-
 xylotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
23.3
-
 xylobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
23.3
-
 cellotriose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
25
-
 xylotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
30
-
 cellobiose mutant enzyme Y300N, at 37°C and pH 8.0 Sarocladium strictum
45
-
 cellotriose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
48.3
-
 cellotriose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
56.7
-
 cellobiose mutant enzyme Y300A, at 37°C and pH 8.0 Sarocladium strictum
68.3
-
 cellobiose mutant enzyme W351F, at 37°C and pH 8.0 Sarocladium strictum
70
-
 cellotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
85
-
 xylotriose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
90
-
 xylobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum
100
-
 cellobiose wild type enzyme, at 37°C and pH 8.0 Sarocladium strictum