BRENDA - Enzyme Database
show all sequences of 1.1.99.B1

Flavin adenine dinucleotide-dependent 4-phospho-D-erythronate dehydrogenase is responsible for the 4-phosphohydroxy-L-threonine pathway in vitamin B6 biosynthesis in Sinorhizobium meliloti

Tazoe, M.; Ichikawa, K.; Hoshino, T.; J. Bacteriol. 188, 4635-4645 (2006)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Sinorhizobium meliloti
53190
-
predicted from the pdxR DNA sequence
Sinorhizobium meliloti
106000
-
gel filtration chromatography
Sinorhizobium meliloti
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sinorhizobium meliloti
-
formerly Rhizobium meliloti, strain IFO 14782
-
Purification (Commentary)
Commentary
Organism
Q Sepharose FF column chromatography and Sephadex LH 20 column chromatography
Sinorhizobium meliloti
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.946
-
0.091 mM DCIP and 0.00024 mg purified enzyme in 33 mM potassium phosphate buffer at pH 7.0
Sinorhizobium meliloti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-phospho-D-erythronate + 2,6-dichloroindophenol +
-
669121
Sinorhizobium meliloti
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + reduced 2,6-dichloroindophenol
-
-
-
?
4-phospho-D-erythronate + FAD
-
669121
Sinorhizobium meliloti
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + FADH2
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 53000, SDS-PAGE
Sinorhizobium meliloti
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Sinorhizobium meliloti
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Sinorhizobium meliloti
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
SDS-PAGE
Sinorhizobium meliloti
53190
-
predicted from the pdxR DNA sequence
Sinorhizobium meliloti
106000
-
gel filtration chromatography
Sinorhizobium meliloti
Purification (Commentary) (protein specific)
Commentary
Organism
Q Sepharose FF column chromatography and Sephadex LH 20 column chromatography
Sinorhizobium meliloti
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.946
-
0.091 mM DCIP and 0.00024 mg purified enzyme in 33 mM potassium phosphate buffer at pH 7.0
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-phospho-D-erythronate + 2,6-dichloroindophenol +
-
669121
Sinorhizobium meliloti
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + reduced 2,6-dichloroindophenol
-
-
-
?
4-phospho-D-erythronate + FAD
-
669121
Sinorhizobium meliloti
3-hydroxy-4-phosphohydroxy-alpha-ketobutyrate + FADH2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 53000, SDS-PAGE
Sinorhizobium meliloti
Other publictions for EC 1.1.99.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
669121
Tazoe
Flavin adenine dinucleotide-de ...
Sinorhizobium meliloti
J. Bacteriol.
188
4635-4645
2006
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