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Literature summary for 1.1.99.6 extracted from

  • Reed, D.W.; Hartzell, P.L.
    The Archaeoglobus fulgidus D-lactate dehydrogenase is a Zn2+ flavoprotein (1999), J. Bacteriol., 181, 7580-7587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.6

Activating Compound

Activating Compound Comment Organism Structure
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate stimulates Archaeoglobus fulgidus
n-dodecyl beta-D-maltoside stimulates Archaeoglobus fulgidus
phenazine methosulfate stimulates Archaeoglobus fulgidus
Triton X-100 stimulates Archaeoglobus fulgidus

Cloned(Commentary)

Cloned (Comment) Organism
expression as part of a fusion protein with maltose-binding protein in Escherichia coli Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
oxalate 1 mM, addition of Tris buffered oxalate to a reaction mixture inhibits activity Archaeoglobus fulgidus
Tris slight inhibition above 30 mM Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 (R)-lactate pH 8.5, 60°C Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme contains 1 mol of Zn2+ per mol of protein Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29853
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + oxidized 2,6-dichlorophenolindophenol no activity with L-lactate Archaeoglobus fulgidus pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
(R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 Archaeoglobus fulgidus pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
-
 ?
additional information methylene blue, dimethylnaphthoquinone, NAD+, and horse heart cytochrome c do not serve as electron acceptors for the purified enzyme Archaeoglobus fulgidus ?
-
 ?

Synonyms

Synonyms Comment Organism
AF0394
-
 Archaeoglobus fulgidus
Dld enzyme
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Archaeoglobus fulgidus
90
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 95 25°C: 8% of maximal activity, 95°C: about 75% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
83
-
 half-life: 105 min Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in a 25 mM Tris buffer at 60°C Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 30% of maximal activit, pH 9.5: about 55% of maximal activity Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein. FAD binds to apoprotein with a 1:1 stoichiometry and is essential for activity. Although the cofactor is tightly associated with the enzyme, it is not covalently attached Archaeoglobus fulgidus