Protein Variants | Comment | Organism |
---|---|---|
E149Q | mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH | Mesorhizobium loti |
H137L | almost complete loss of activity | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0124 | - |
NADH | wild-type, pH 8.0, 30°C | Mesorhizobium loti | |
0.02 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | mutant E149Q, pH 8.0, 30°C | Mesorhizobium loti | |
0.0249 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | wild-type, pH 8.0, 30°C | Mesorhizobium loti | |
0.026 | - |
NADH | mutant E149Q, pH 8.0, 30°C | Mesorhizobium loti | |
0.03 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | mutant E149Q, pH 5.0, 30°C | Mesorhizobium loti | |
0.033 | - |
NADH | mutant E149Q, pH 5.0, 30°C | Mesorhizobium loti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59100 | - |
gel filtration | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | Q988H5 | enzyme additionally catalyzes the reaction of EC 1.2.1.100 | - |
Mesorhizobium loti MAFF303099 | Q988H5 | enzyme additionally catalyzes the reaction of EC 1.2.1.100 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+ | - |
Mesorhizobium loti | 4-pyridoxate + NAD+ | - |
? | |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+ | - |
Mesorhizobium loti MAFF303099 | 4-pyridoxate + NAD+ | - |
? | |
additional information | enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction | Mesorhizobium loti | ? | - |
? | |
additional information | enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction | Mesorhizobium loti MAFF303099 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
mlr6739 | - |
Mesorhizobium loti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | mutant E149Q, pH 8.0, 30°C | Mesorhizobium loti | |
217 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | wild-type, pH 8.0, 30°C | Mesorhizobium loti | |
300 | - |
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate | mutant E149Q, pH 5.0, 30°C | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mesorhizobium loti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mesorhizobium loti |
General Information | Comment | Organism |
---|---|---|
physiological function | contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source | Mesorhizobium loti |