KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
D-2-hydroxyglutarate | pH 9.5, 30°C | Saccharomyces cerevisiae | |
0.45 | - |
pyruvate | pH 9.5, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.005 mM, about 3fold stimulation | Saccharomyces cerevisiae | |
Zn2+ | 0.005 mM, about 3fold stimulation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P39976 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein. Purified fractions show a yellow color | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + D-lactate | - |
Saccharomyces cerevisiae | D-2-hydroxyglutarate + pyruvate | - |
r | |
D-2-hydroxyglutarate + oxaloacetate | - |
Saccharomyces cerevisiae | 2-oxoglutarate + malate | - |
r | |
D-2-hydroxyglutarate + pyruvate | - |
Saccharomyces cerevisiae | 2-oxoglutarate + D-lactate | - |
r | |
additional information | no substrate: L-lactate. The enzyme displays dehydrogenase activity with artificial acceptor dichlorophenolindophenol | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-2-hydroxyglutarate-pyruvate transhydrogenase | - |
Saccharomyces cerevisiae |
DLD3 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
D-2-hydroxyglutarate | pH 9.5, 30°C | Saccharomyces cerevisiae | |
4.9 | - |
pyruvate | pH 9.5, 30°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme is a FAD-dependent transhydrogenase using pyruvate as a hydrogen acceptor | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | a mutant strain lacking cytosolic transhydrogenase Dld3 activity accumulates millimolar levels of D-2-hydroxyglutarate | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
pyruvate | pH 9.5, 30°C | Saccharomyces cerevisiae | |
36 | - |
D-2-hydroxyglutarate | pH 9.5, 30°C | Saccharomyces cerevisiae |