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Literature summary for 1.1.99.39 extracted from

  • Engqvist, M.; Drincovich, M.F.; Fluegge, U.I.; Maurino, V.G.
    Two D-2-hydroxy-acid dehydrogenases in Arabidopsis thaliana with catalytic capacities to participate in the last reactions of the methylglyoxal and beta-oxidation pathways (2009), J. Biol. Chem., 284, 25026-25037.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.584
-
 (R)-2-hydroxyglutarate 25°C, pH 8.75, cofactor oxidized 2,6-dichlorophenolindophenol Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61450
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-hydroxyglutarate + acceptor Arabidopsis thaliana
-
 2-oxoglutarate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O23240
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutarate + acceptor
-
 Arabidopsis thaliana 2-oxoglutarate + reduced acceptor
-
 ?
(R)-2-hydroxyglutarate + oxidized 2,6-dichlorophenolindophenol
-
 Arabidopsis thaliana 2-oxoglutarate + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information low activity with D-lactate, D-2-hydroxybutyrate, meso-tartrate Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
AtD-2HGDH
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 (R)-2-hydroxyglutarate 25°C, pH 8.75, cofactor oxidized 2,6-dichlorophenolindophenol Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
additional information no reaction with NAD+, NADP+ or cytochrome c Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.37
-
 (R)-2-hydroxyglutarate 25°C, pH 8.75, cofactor oxidized 2,6-dichlorophenolindophenol Arabidopsis thaliana