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Literature summary for 1.1.99.35 extracted from
- Monitoring of PQQ-dependent glucose dehydrogenase substrate specificity for its potential use in biocatalysis and bioanalysis (2013), Appl. Biochem. Biotechnol., 171, 1032-1041.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | cellobiono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? |  |
| D-allose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-galactose + N-methylphenazonium methylsulfate | 30% activity compared to D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-glucose + N-methylphenazonium methylsulfate | 100% activity | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-lyxose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-lyxono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-mannose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-ribose + N-methylphenazonium methylsulfate | 5% activity compared to D-glucose | Acinetobacter calcoaceticus | D-ribono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| D-xylose + N-methylphenazonium methylsulfate | 14% activity compared to D-glucose | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| L-arabinose + N-methylphenazonium methylsulfate | - |
Acinetobacter calcoaceticus | L-arabinono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| lactose + N-methylphenazonium methylsulfate | 4% activity compared to D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| maltose + N-methylphenazonium methylsulfate | 12% activity compared to D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| melibiose + N-methylphenazonium methylsulfate | 5% activity compared to D-glucose | Acinetobacter calcoaceticus | melibiono-1,5-lactone + reduced N-methylphenazonium methylsulfate | - |
? | |
| additional information | no activity with D-glucosamine, L-altrose, L-lyxose, L-talose, L-mannose, D-altrose, D-arabinose, D-talose, L-xylose, L-glucose, L-galactose, L-allose, sucrose, trehalose, and raffinose | Acinetobacter calcoaceticus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,7,9-tricarboxypyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| sGDH-PQQ | - |
Acinetobacter calcoaceticus |
| soluble PQQ-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
D-Lyxose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 11 | - |
D-ribose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 11 | - |
melibiose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 46 | - |
D-xylose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 50 | - |
D-mannose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 150 | - |
D-galactose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 790 | - |
lactose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 800 | - |
maltose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1080 | - |
cellobiose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1450 | - |
L-arabinose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1470 | - |
D-allose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
| 1700 | - |
D-glucose | free enzyme, pH and temperature not specified in the publication | Acinetobacter calcoaceticus | |
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