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Literature summary for 1.1.99.35 extracted from
- High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli (2012), Eng. Life Sci., 12, 574-582.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acinetobacter calcoaceticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Acinetobacter calcoaceticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 13% activity compared to D-glucose | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |  |
| D-galactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | about 13% activity compared to D-glucose | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | 100% activity | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | 100% activity | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-mannose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 11% activity compared to D-glucose | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 9% activity compared to D-glucose | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 50% activity compared to D-glucose | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 70% activity compared to D-glucose | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | Acinetobacter calcoaceticus | almost no activity with D-fructose | ? | - |
? | |
| additional information | Acinetobacter calcoaceticus L.M.D. 79.41 | almost no activity with D-fructose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
| Acinetobacter calcoaceticus L.M.D. 79.41 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity column chromatography | Acinetobacter calcoaceticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:D-glucose-grown cell | - |
Acinetobacter calcoaceticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 515 | - |
crude cell lysate, at pH 7.0 and 25°C | Acinetobacter calcoaceticus |
| 5811 | - |
after 11.3fold purification, at pH 7.0 and 25°C | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + oxidized 2,6-dichlorophenolindophenol | about 13% activity compared to D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-galactose + oxidized 2,6-dichlorophenolindophenol | about 13% activity compared to D-glucose | Acinetobacter calcoaceticus L.M.D. 79.41 | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | 100% activity | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | 100% activity | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-mannose + oxidized 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + oxidized 2,6-dichlorophenolindophenol | about 9% activity compared to D-glucose | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + oxidized 2,6-dichlorophenolindophenol | about 50% activity compared to D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + oxidized 2,6-dichlorophenolindophenol | about 70% activity compared to D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | almost no activity with D-fructose | Acinetobacter calcoaceticus | ? | - |
? | |
| additional information | almost no activity with D-fructose | Acinetobacter calcoaceticus L.M.D. 79.41 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQGDH | - |
Acinetobacter calcoaceticus |
| soluble pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the half-life of enzyme thermal inactivation at 55°C is 12.1 min | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
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