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Literature summary for 1.1.99.35 extracted from

  • Tanaka, S.; Igarashi, S.; Ferri, S.; Sode, K.
    Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface (2005), BMC Biochem., 6, 1.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.35

Protein Variants

Protein Variants Comment Organism
D340F/Y418F increase in temperature stability Acinetobacter calcoaceticus
D340F/Y418I increase in temperature stability Acinetobacter calcoaceticus
T416V/T417V increase in temperature stability Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16
-
 D-glucose mutant T416V/T417V, pH 7.0, 25°C Acinetobacter calcoaceticus
20
-
 D-glucose wild-type, pH 7.0, 25°C Acinetobacter calcoaceticus
20
-
 D-glucose mutant D340F/Y418F, pH 7.0, 25°C Acinetobacter calcoaceticus
20
-
 D-glucose mutant D340F/Y418I, pH 7.0, 25°C Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53.9
-
 wild-type, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
55
-
 half-life of wild-type, 9.5 min, of mutant D340F/Y418F 14.9 min, of mutant D340F/Y418I 15.5 min, of mutant T416V/T417V 14.8 min, respectively Acinetobacter calcoaceticus
56.5
-
 mutant T416V/T417V, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
57.5
-
 mutant D340F/Y418I, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus
57.7
-
 mutant D340F/Y418F, 50% loss of initial activity within in 10 min Acinetobacter calcoaceticus