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Literature summary for 1.1.99.35 extracted from
- On the mechanism and specificity of soluble, quinoprotein glucose dehydrogenase in the oxidation of aldose sugars (1998), Biochemistry, 37, 13854-13861.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |  |
| 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| cellobiose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-allose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-fucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-fucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-galactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-lyxose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-lyxono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-mannose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-ribose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| L-arabinose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | L-arabinono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| melibiose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| additional information | kinetic mechanism of sGDH consists of (a) step(s) in which a fluorescing intermediate is formed, and a subsequent, irreversible step, determining the overall rate of the reductive half-reaction. The pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions. | Acinetobacter calcoaceticus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sGDH | - |
Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
pH optimum determined for glucose oxidation appears to be 7.0, implying that reoxidation of sGDHred is rate-limiting with those electron acceptors displaying a different value under steady-state conditions | Acinetobacter calcoaceticus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
D-Lyxose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 11 | - |
D-ribose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 11 | - |
melibiose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 45 | - |
D-xylose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
| 46 | - |
D-xylose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 50 | - |
D-mannose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 150 | - |
D-galactose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 240 | - |
D-galactose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
| 440 | - |
D-fucose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 790 | - |
lactose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 800 | - |
maltose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 960 | - |
lactose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
| 1080 | - |
cellobiose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 1450 | - |
L-arabinose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 1470 | - |
D-allose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 1700 | - |
D-glucose | stopped-flow measurement of reductive half-reaction, 15°C | Acinetobacter calcoaceticus | |
| 2100 | - |
D-glucose | steady-state kinetics, 20°C | Acinetobacter calcoaceticus | |
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