Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone | Gluconobacter oxydans | |
Tween 80 | solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.8 mM, complete loss of activity | Gluconobacter oxydans | |
EDTA | 5.2 mM, 13% inhibition | Gluconobacter oxydans | |
iodoacetate | 4.2 mM, 34% inhibition | Gluconobacter oxydans | |
Quinine | 4.2 mM, 30% inhibition | Gluconobacter oxydans | |
sodium fluoroacetate | 9.4 mM, 26% inhibition | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
L-sorbose | electron acceptor 2,6-dichloroindophenol, pH 7.0, 25°C | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter oxydans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.8 mM, 18% increase in activity | Gluconobacter oxydans | |
Fe3+ | 0.4 mM, 21% increase in activity | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, SDS-PAGE | Gluconobacter oxydans |
210000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
enzyme activity is induced by growth on L-sorbose or D-sorbitol as carbon source | - |
Gluconobacter oxydans UV10 | - |
enzyme activity is induced by growth on L-sorbose or D-sorbitol as carbon source | - |
Purification (Comment) | Organism |
---|---|
purification from membrane fraction | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.897 | - |
pH 7.0, 25°C | Gluconobacter oxydans |
Storage Stability | Organism |
---|---|
rather stable in buffer containing 200 mM sorbose, pH 7.0, below 30°C | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-sorbose + 2,6-dichloroindophenol | - |
Gluconobacter oxydans | L-sorbosone + reduced 2,6-dichloroindophenol | - |
? | |
L-sorbose + 2,6-dichloroindophenol | - |
Gluconobacter oxydans UV10 | L-sorbosone + reduced 2,6-dichloroindophenol | - |
? | |
L-sorbose + phenazine methosulfate | - |
Gluconobacter oxydans | L-sorbosone + reduced phenazine methosulfate | - |
? | |
L-sorbose + phenazine methosulfate | - |
Gluconobacter oxydans UV10 | L-sorbosone + reduced phenazine methosulfate | - |
? | |
additional information | enzyme is highly specific for L-sorbose. No substrates are glucose, mannitol, sorbitol, fructose, glycerol, adonitol, erythritol, gluconate, calcium idonate, mannose, maltose, xylose, ethanol, lactose, sucrose, galactose, cellobiose, inositol, arabinose, sodium 2-keto-L-gulonic acid | Gluconobacter oxydans | ? | - |
? | |
additional information | enzyme is highly specific for L-sorbose. No substrates are glucose, mannitol, sorbitol, fructose, glycerol, adonitol, erythritol, gluconate, calcium idonate, mannose, maltose, xylose, ethanol, lactose, sucrose, galactose, cellobiose, inositol, arabinose, sodium 2-keto-L-gulonic acid | Gluconobacter oxydans UV10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
L-sorbose dehydrogenase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
- |
Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable up to | Gluconobacter oxydans |
46 | - |
5 min, 50% loss of activity | Gluconobacter oxydans |
65 | - |
5 min, 90% loss of activity | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
unstable below | Gluconobacter oxydans |
6.5 | 8 | - |
Gluconobacter oxydans |