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Literature summary for 1.1.99.32 extracted from

  • Sugisawa, T.; Hoshino, T.; Nomura, S.; Fujiwara, A.
    Isolation and characterization of membrane-bound L-sorbose dehydrogenase from Gluconobacter melanogenus UV10 (1991), Agric. Biol. Chem., 55, 363-370.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone Gluconobacter oxydans
Tween 80 solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.8 mM, complete loss of activity Gluconobacter oxydans
EDTA 5.2 mM, 13% inhibition Gluconobacter oxydans
iodoacetate 4.2 mM, 34% inhibition Gluconobacter oxydans
Quinine 4.2 mM, 30% inhibition Gluconobacter oxydans
sodium fluoroacetate 9.4 mM, 26% inhibition Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
100
-
L-sorbose electron acceptor 2,6-dichloroindophenol, pH 7.0, 25°C Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Gluconobacter oxydans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.8 mM, 18% increase in activity Gluconobacter oxydans
Fe3+ 0.4 mM, 21% increase in activity Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
4 * 58000, SDS-PAGE Gluconobacter oxydans
210000
-
gel filtration Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
enzyme activity is induced by growth on L-sorbose or D-sorbitol as carbon source
-
Gluconobacter oxydans UV10
-
enzyme activity is induced by growth on L-sorbose or D-sorbitol as carbon source
-

Purification (Commentary)

Purification (Comment) Organism
purification from membrane fraction Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.897
-
pH 7.0, 25°C Gluconobacter oxydans

Storage Stability

Storage Stability Organism
rather stable in buffer containing 200 mM sorbose, pH 7.0, below 30°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-sorbose + 2,6-dichloroindophenol
-
Gluconobacter oxydans L-sorbosone + reduced 2,6-dichloroindophenol
-
?
L-sorbose + 2,6-dichloroindophenol
-
Gluconobacter oxydans UV10 L-sorbosone + reduced 2,6-dichloroindophenol
-
?
L-sorbose + phenazine methosulfate
-
Gluconobacter oxydans L-sorbosone + reduced phenazine methosulfate
-
?
L-sorbose + phenazine methosulfate
-
Gluconobacter oxydans UV10 L-sorbosone + reduced phenazine methosulfate
-
?
additional information enzyme is highly specific for L-sorbose. No substrates are glucose, mannitol, sorbitol, fructose, glycerol, adonitol, erythritol, gluconate, calcium idonate, mannose, maltose, xylose, ethanol, lactose, sucrose, galactose, cellobiose, inositol, arabinose, sodium 2-keto-L-gulonic acid Gluconobacter oxydans ?
-
?
additional information enzyme is highly specific for L-sorbose. No substrates are glucose, mannitol, sorbitol, fructose, glycerol, adonitol, erythritol, gluconate, calcium idonate, mannose, maltose, xylose, ethanol, lactose, sucrose, galactose, cellobiose, inositol, arabinose, sodium 2-keto-L-gulonic acid Gluconobacter oxydans UV10 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
L-sorbose dehydrogenase
-
Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
48
-
-
Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable up to Gluconobacter oxydans
46
-
5 min, 50% loss of activity Gluconobacter oxydans
65
-
5 min, 90% loss of activity Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
unstable below Gluconobacter oxydans
6.5 8
-
Gluconobacter oxydans