Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
(S)-Mandelate | at pH 6.5 and 30°C | Pseudomonas aeruginosa | |
3.87 | - |
ferricyanide | at pH 6.5 and 30°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate + 2 ferricyanide | Pseudomonas aeruginosa | - |
benzoylformic acid + 2 ferrocyanide + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl DEAE-650M column chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.68 | - |
crude extract, at pH 6.5 and 30°C | Pseudomonas aeruginosa |
20.5 | - |
after 7.65fold purification, at pH 6.5 and 30°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate + 2 ferricyanide | - |
Pseudomonas aeruginosa | benzoylformic acid + 2 ferrocyanide + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMDH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | about 58% activity at 20°C, about 50% activity at 40°C | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the remaining activity after treatment in 50 mM potassium phosphate buffer (pH 6.5) at 30°C was 92% for 15 h | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas aeruginosa |