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Literature summary for 1.1.99.31 extracted from

  • Sukumar, N.; Dewanti, A.; Merli, A.; Rossi, G.L.; Mitra, B.; Mathews, F.S.
    Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates (2009), Acta Crystallogr. Sect. D, 65, 543-552.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.31

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant G81A of the catalytically similar, soluble chimera MDH-GOX2 (mandelate dehydrogenase/glycolate oxidase) Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
G81A mutation in the catalytically similar chimera MDH-GOX2 (mandelate dehydrogenase/glycolate oxidase), mutant has lower reactivity with substrate Pseudomonas putida
G81A wild type, mutant has lower reactivity with substrate Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 Mandelate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
0.09
-
 Mandelate chimera MDH-GOX2 Pseudomonas putida
0.13
-
 3-indolelactate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
0.14
-
 2-Hydroxyoctanoate mutant G81A Pseudomonas putida
0.17
-
 3-indolelactate mutant G81A Pseudomonas putida
0.24
-
 Mandelate wild-type enzyme Pseudomonas putida
0.24
-
 Mandelate mutant G81A Pseudomonas putida
0.29
-
 2-Hydroxyoctanoate chimera MDH-GOX2 Pseudomonas putida
0.57
-
 2-Hydroxyoctanoate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
0.64
-
 3-indolelactate chimera MDH-GOX2 Pseudomonas putida
0.8
-
 2-Hydroxyoctanoate wild-type enzyme Pseudomonas putida
0.9
-
 3-indolelactate wild-type enzyme Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20932
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate + acceptor
-
 Pseudomonas putida benzoylformate + reduced acceptor
-
 ?
2-hydroxyoctanoate + acceptor
-
 Pseudomonas putida ? + reduced acceptor
-
 ?
3-indolelactate + acceptor
-
 Pseudomonas putida ? + reduced acceptor
-
 ?
mandelate + acceptor
-
 Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
(S)-mandelate dehydrogenase
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 2-Hydroxyoctanoate mutant G81A Pseudomonas putida
0.3
-
 3-indolelactate mutant G81A Pseudomonas putida
0.42
-
 2-Hydroxyoctanoate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
0.5
-
 2-Hydroxyoctanoate wild-type enzyme Pseudomonas putida
1
-
 3-indolelactate wild-type enzyme Pseudomonas putida
1
-
 2-Hydroxyoctanoate chimera MDH-GOX2 Pseudomonas putida
1.4
-
 3-indolelactate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
2.3
-
 Mandelate mutant G81A of chimera MDH-GOX2 Pseudomonas putida
3.9
-
 3-indolelactate chimera MDH-GOX2 Pseudomonas putida
15.2
-
 Mandelate mutant G81A Pseudomonas putida
205
-
 Mandelate chimera MDH-GOX2 Pseudomonas putida
350
-
 Mandelate wild-type enzyme Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Pseudomonas putida