Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
mutant G81A of the catalytically similar, soluble chimera MDH-GOX2 (mandelate dehydrogenase/glycolate oxidase) | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
G81A | mutation in the catalytically similar chimera MDH-GOX2 (mandelate dehydrogenase/glycolate oxidase), mutant has lower reactivity with substrate | Pseudomonas putida |
G81A | wild type, mutant has lower reactivity with substrate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Mandelate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
0.09 | - |
Mandelate | chimera MDH-GOX2 | Pseudomonas putida | |
0.13 | - |
3-indolelactate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
0.14 | - |
2-Hydroxyoctanoate | mutant G81A | Pseudomonas putida | |
0.17 | - |
3-indolelactate | mutant G81A | Pseudomonas putida | |
0.24 | - |
Mandelate | wild-type enzyme | Pseudomonas putida | |
0.24 | - |
Mandelate | mutant G81A | Pseudomonas putida | |
0.29 | - |
2-Hydroxyoctanoate | chimera MDH-GOX2 | Pseudomonas putida | |
0.57 | - |
2-Hydroxyoctanoate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
0.64 | - |
3-indolelactate | chimera MDH-GOX2 | Pseudomonas putida | |
0.8 | - |
2-Hydroxyoctanoate | wild-type enzyme | Pseudomonas putida | |
0.9 | - |
3-indolelactate | wild-type enzyme | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20932 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate + acceptor | - |
Pseudomonas putida | benzoylformate + reduced acceptor | - |
? | |
2-hydroxyoctanoate + acceptor | - |
Pseudomonas putida | ? + reduced acceptor | - |
? | |
3-indolelactate + acceptor | - |
Pseudomonas putida | ? + reduced acceptor | - |
? | |
mandelate + acceptor | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(S)-mandelate dehydrogenase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-Hydroxyoctanoate | mutant G81A | Pseudomonas putida | |
0.3 | - |
3-indolelactate | mutant G81A | Pseudomonas putida | |
0.42 | - |
2-Hydroxyoctanoate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
0.5 | - |
2-Hydroxyoctanoate | wild-type enzyme | Pseudomonas putida | |
1 | - |
3-indolelactate | wild-type enzyme | Pseudomonas putida | |
1 | - |
2-Hydroxyoctanoate | chimera MDH-GOX2 | Pseudomonas putida | |
1.4 | - |
3-indolelactate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
2.3 | - |
Mandelate | mutant G81A of chimera MDH-GOX2 | Pseudomonas putida | |
3.9 | - |
3-indolelactate | chimera MDH-GOX2 | Pseudomonas putida | |
15.2 | - |
Mandelate | mutant G81A | Pseudomonas putida | |
205 | - |
Mandelate | chimera MDH-GOX2 | Pseudomonas putida | |
350 | - |
Mandelate | wild-type enzyme | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas putida |