Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach is soluble and retains partial catalytic activity (about 1%) using (S)-mandelate as substrate. The activities of the soluble mutant enzymes (S)-mandelate dehydrogenase with residues 2-4 deleted and (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus are nearly the same when (S)-phenyllactate is used as substrate | Pseudomonas putida |
General Stability | Organism |
---|---|
repeated freeze-thaw cycles cause significant loss of activity | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.158 | - |
(S)-Mandelate | pH 7.5, (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus | Pseudomonas putida | |
0.206 | - |
(S)-Mandelate | pH 7.5, wild-type enzyme | Pseudomonas putida | |
0.225 | - |
(S)-Mandelate | pH 7.5, (S)-mandelate dehydrogenase with residues 2-4 deleted | Pseudomonas putida | |
0.229 | - |
(S)-Mandelate | pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach | Pseudomonas putida | |
0.78 | - |
(S)-phenyllactate | pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach | Pseudomonas putida | |
2.6 | - |
(S)-phenyllactate | pH 7.5, wild-type enzyme | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated, wild-type enzyme | Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-70°C, wild-type enzyme can be stored frozen in 20% ethanediol for weeks without loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(S)-phenyllactate + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
(S)-Mandelate | pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach | Pseudomonas putida | |
9 | - |
(S)-Mandelate | pH 7.5, (S)-mandelate dehydrogenase with residues 2-4 deleted | Pseudomonas putida | |
24 | - |
(S)-Mandelate | pH 7.5, (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus | Pseudomonas putida | |
174 | - |
(S)-Mandelate | pH 7.5, wild-type enzyme | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Pseudomonas putida |