KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
3-hydroxy-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.12 | - |
3-methoxy-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.12 | - |
4-methoxy-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.15 | - |
4-chloro-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.16 | - |
4-fluoro-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.17 | - |
4-methyl-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.24 | - |
L-Mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.26 | - |
4-bromo-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.35 | - |
D,L-Mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.38 | - |
4-chloro-D,L-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
0.74 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula graminis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(3-hydroxyphenyl)acetate + ferrocyanide + H2O | - |
? | |
3-methoxy-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(3-methoxyphenyl)acetate + ferrocyanide + H2O | - |
? | |
4-bromo-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-bromophenyl)acetate + ferrocyanide + H2O | - |
? | |
4-chloro-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-chlorophenyl)acetate + ferrocyanide + H2O | - |
? | |
4-fluoro-D,L-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-fluorophenyl)acetate + ferrocyanide + H2O | - |
? | |
4-hydroxy-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-hydroxyphenyl)acetate + ferrocyanide + H2O | - |
? | |
4-methoxy-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-methoxyphenyl)acetate + ferrocyanide + H2O | - |
? | |
4-methyl-DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-(4-methylphenyl)acetate + ferrocyanide + H2O | - |
? | |
DL-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ferrocyanide + H2O | - |
? | |
L-mandelate + ferricyanide | - |
Rhodotorula graminis | 2-oxo-2-phenylacetate + ferrocyanide | - |
? | |
additional information | enzyme-catalyzed reaction proceeds via the same transition state for each substrate and indicates that this transition state is relatively non-polar but has an electron-rich centre at the alpha-carbon position | Rhodotorula graminis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68 | - |
4-chloro-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
68 | - |
3-methoxy-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
80 | - |
4-methyl-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
93 | - |
DL-[2-2H]-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
94 | - |
D,L-Mandelate | pH 7.5, 25°C, cosubstrate: ferricyanid | Rhodotorula graminis | |
98 | - |
4-fluoro-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
106 | - |
4-methoxy-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
108 | - |
4-bromo-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
108 | - |
3-hydroxy-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
114 | - |
L-Mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
116 | - |
4-chloro-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis | |
146 | - |
4-hydroxy-DL-mandelate | pH 7.5, 25°C, cosubstrate: ferricyanide | Rhodotorula graminis |